Values CatLog read from this paper
Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain
PMID 15894617doi:10.1073/pnas.0501003102Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 23 records (30 values) from this paper: tRNAIle-lysidine synthetase (TilS) from Aquifex aeolicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tRNAIle-lysidine synthetase (TilS) | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.0194 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS), adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS) | Aquifex aeolicus | L-lysine | 0.1563 | 0.6294 | 0.2483 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS), L-lysine |
| tRNAIle-lysidine synthetase (TilS) | Aquifex aeolicus | tRNAIle2 transcript | — | 0.00334 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS), tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant E106A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.0385 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E106A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant E106A | Aquifex aeolicus | L-lysine | 0.0085 | 1.399 | 0.0061 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E106A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant E106A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.0019 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E106A, tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant E140A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.1346 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E140A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant E140A | Aquifex aeolicus | L-lysine | 0.0277 | 1.364 | 0.0203 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E140A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant E140A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.01567 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant E140A, tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant H133A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.00767 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant H133A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant H133A | Aquifex aeolicus | L-lysine | 0.125 | 1.038 | 0.120 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant H133A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant H133A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.00354 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant H133A, tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant R174A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.0412 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R174A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant R174A | Aquifex aeolicus | L-lysine | 0.1738 | 1.147 | 0.1515 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R174A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant R174A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.0202 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R174A, tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant R205A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.2872 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R205A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant R205A | Aquifex aeolicus | L-lysine | 0.00957 | 1.013 | 0.00945 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R205A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant R205A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.0119 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant R205A, tRNAIle2 transcript |
| tRNAIle-lysidine synthetase (TilS)variant S37A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.255 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant S37A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant W188A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.097 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant W188A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant Y114A | Aquifex aeolicus | adenosine 5'-triphosphate | — | 0.1551 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant Y114A, adenosine 5'-triphosphate |
| tRNAIle-lysidine synthetase (TilS)variant Y114A | Aquifex aeolicus | L-lysine | 0.0517 | 3.053 | 0.0169 calc. | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant Y114A, L-lysine |
| tRNAIle-lysidine synthetase (TilS)variant Y114A | Aquifex aeolicus | tRNAIle2 transcript | — | 0.00736 | — | pH 7.8, 60 °C | Open record: tRNAIle-lysidine synthetase (TilS) variant Y114A, tRNAIle2 transcript |