CatLogenzyme kinetics catalog

Values CatLog read from this paper

The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate

PMID 15896703doi:10.1016/j.abb.2005.04.011Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 51 records (75 values) from this paper: human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1, human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2, bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (BHP) and 1 more from Homo sapiens, Bos taurus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.1.105, EC 3.1.3.46

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (BHP)Bos taurusfructose 6-phosphate0.4090.017723.1 calc.pH 7.5, 30 °COpen
bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (BHP)Bos taurusfructose 6-phosphate0.2850.04975.73 calc.pH 7.5, 30 °COpen
bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (BHP)variant D499A/E502A/D505ABos taurusfructose 6-phosphate0.30.022413 calc.pH 7.5, 30 °COpen
bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (BHP)variant D499A/E502A/D505ABos taurusfructose 6-phosphate0.2090.05114.09 calc.pH 7.5, 30 °COpen
HBPBHP chimera (human brain HBP1 residues 1-444 fused to bovine heart BHP C-terminal region residues 450-530)chimera HBPBHP—fructose 6-phosphate0.6780.013151.8 calc.pH 7.5, 30 °COpen
HBPBHP chimera (human brain HBP1 residues 1-444 fused to bovine heart BHP C-terminal region residues 450-530)chimera HBPBHP—fructose 6-phosphate0.6540.01350 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensadenosine 5′-triphosphate2-500 µM ATP and 2-5000 µM Fru-6-P both varied; in the text—0.0508—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensadenosine 5′-triphosphate10-500 µM ATP varied; 1 mM Fru-6-P; Table 4—0.0436—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensadenosine 5′-triphosphate10-500 µM ATP varied; 1 mM Fru-6-P; Table 4—0.0469—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensadenosine 5′-triphosphate10-500 µM ATP varied; 1 mM Fru-6-P; Table 4—0.0648—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensadenosine 5′-triphosphate10-500 µM ATP varied; 1 mM Fru-6-P; Table 4—0.0475—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphate2-500 µM ATP and 2-5000 µM Fru-6-P both varied; in the text0.9220.011282.3 calc.pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphate5 nM enzyme dimer; 20 min; Table 30.4070.007157 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphate5 nM enzyme dimer; 20 min; expressed in E. coli; Table 30.430.00854 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphateTable 40.5850.008370 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphateTable 40.5550.007871 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphateTable 40.5710.009858 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphateTable 40.5320.008761 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphatefixed 5 mM ATP and 1 mM Fru-6-P; 0-500 µM PEP varied0.716——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphatefixed 5 mM ATP and 1 mM Fru-6-P; 0-500 µM PEP varied0.691——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphatefixed 5 mM ATP and 1 mM Fru-6-P; 0-500 µM PEP varied0.742——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensfructose 6-phosphatefixed 5 mM ATP and 1 mM Fru-6-P; 0-500 µM PEP varied0.718——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensβ-D-fructose 2,6-bisphosphate2500 nM enzyme dimer; 60 min; Table 31.13 × 10⁻⁴——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensβ-D-fructose 2,6-bisphosphate2500 nM enzyme dimer; 60 min; expressed in E. coli; Table 36.2 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensβ-D-fructose 2,6-bisphosphateTable 41.11 × 10⁻⁴——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1Homo sapiensβ-D-fructose 2,6-bisphosphateTable 49.7 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant H253AHomo sapiensfructose 6-phosphate0.3910.007354 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant H253AHomo sapiensβ-D-fructose 2,6-bisphosphate2.22 × 10⁻⁴——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant H253A/S460DHomo sapiensfructose 6-phosphate0.4010.007156 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant H253A/S460DHomo sapiensβ-D-fructose 2,6-bisphosphate1.92 × 10⁻⁴——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S302RHomo sapiensfructose 6-phosphate0.3990.009542 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S302RHomo sapiensβ-D-fructose 2,6-bisphosphate0.0662——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S460DHomo sapiensfructose 6-phosphate0.3940.006759 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S460DHomo sapiensfructose 6-phosphate0.808——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S460DHomo sapiensβ-D-fructose 2,6-bisphosphate7.8 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S460D/T470DHomo sapiensfructose 6-phosphate0.3940.006660 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S460D/T470DHomo sapiensβ-D-fructose 2,6-bisphosphate8.5 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S477DHomo sapiensfructose 6-phosphate0.4050.006463 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant S477DHomo sapiensβ-D-fructose 2,6-bisphosphate7.8 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant T470DHomo sapiensfructose 6-phosphate0.4350.006666 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant T470DHomo sapiensβ-D-fructose 2,6-bisphosphate7.7 × 10⁻⁵——pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant ΔC (Δ446-519)/S445THomo sapiensfructose 6-phosphate0.6960.009276 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP1variant ΔC (Δ446-519)/S445THomo sapiensfructose 6-phosphate0.807——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensadenosine 5′-triphosphate—0.0848—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensadenosine 5′-triphosphate—0.0684—pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensfructose 6-phosphate5 nM enzyme dimer; 20 min; Table 30.4470.007460 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensfructose 6-phosphateTable 40.5950.015638.1 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensfructose 6-phosphateTable 40.6070.011552.8 calc.pH 7.5, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensfructose 6-phosphate0.906——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensfructose 6-phosphate0.961——pH 7, 30 °COpen
human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase splice variant HBP2Homo sapiensβ-D-fructose 2,6-bisphosphate4.77 × 10⁻⁴——pH 7.5, 30 °COpen