Values CatLog read from this paper
Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction
PMID 15966723doi:10.1021/bi050583vOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (38 values) from this paper: GDP-mannose mannosyl hydrolase (GDPMH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GDP-mannose mannosyl hydrolase (GDPMH) | Escherichia coli | GDP-2-deoxy-2-fluoro-α-D-mannose | 0.0174 | 0.76 | 0.023 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH), GDP-2-deoxy-2-fluoro-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH) | Escherichia coli | GDP-α-D-glucose | 1.19 | 0.87 | 1.4 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH), GDP-α-D-glucose |
| GDP-mannose mannosyl hydrolase (GDPMH) | Escherichia coli | GDP-α-D-mannose | 0.33 | 0.67 | 0.49 calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH), GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH) | Escherichia coli | GDP-α-D-mannose | 0.28 | 0.3 | 0.93 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH), GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH) | Escherichia coli | GDP-β-L-fucose | 0.0048 | 0.046 | 0.10 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH), GDP-β-L-fucose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22A | Escherichia coli | GDP-2-deoxy-2-fluoro-α-D-mannose | 0.0039 | 0.44 | 0.0089 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22A, GDP-2-deoxy-2-fluoro-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22A | Escherichia coli | GDP-α-D-glucose | 0.0028 | 3.7 | 7.6 × 10⁻⁴ calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22A, GDP-α-D-glucose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22A | Escherichia coli | GDP-α-D-mannose | 0.0027 | 3 | 9.0 × 10⁻⁴ calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22A, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22A | Escherichia coli | GDP-β-L-fucose | 6.8 × 10⁻⁴ | 0.23 | 0.0030 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22A, GDP-β-L-fucose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22N | Escherichia coli | GDP-2-deoxy-2-fluoro-α-D-mannose | 3.7 × 10⁻⁴ | 0.26 | 0.0014 calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22N, GDP-2-deoxy-2-fluoro-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22N | Escherichia coli | GDP-α-D-glucose | 6.8 × 10⁻⁴ | 2.2 | 3.1 × 10⁻⁴ calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22N, GDP-α-D-glucose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22N | Escherichia coli | GDP-α-D-mannose | 8 × 10⁻⁴ | 5.8 | 1.4 × 10⁻⁴ calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22N, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant D22N | Escherichia coli | GDP-β-L-fucose | 1.2 × 10⁻⁴ | 0.22 | 5.5 × 10⁻⁴ calc. | pH 7.2, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant D22N, GDP-β-L-fucose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant H124E | Escherichia coli | GDP-α-D-mannose | 0.002 | 2.7 | 7.4 × 10⁻⁴ calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant H124E, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant H124E/Y127F | Escherichia coli | GDP-α-D-mannose | 0.0058 | 2.3 | 0.0025 calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant H124E/Y127F, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant R37Q | Escherichia coli | GDP-α-D-mannose | 0.0138 | 5.7 | 0.0024 calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant R37Q, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant R37Q/Y103F | Escherichia coli | GDP-α-D-mannose | 0.00175 | 3 | 5.8 × 10⁻⁴ calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant R37Q/Y103F, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant Y103F | Escherichia coli | GDP-α-D-mannose | 0.0032 | 4.6 | 7.0 × 10⁻⁴ calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant Y103F, GDP-α-D-mannose |
| GDP-mannose mannosyl hydrolase (GDPMH)variant Y127F | Escherichia coli | GDP-α-D-mannose | 0.18 | 0.33 | 0.55 calc. | pH 9.3, 37 °C | Open record: GDP-mannose mannosyl hydrolase (GDPMH) variant Y127F, GDP-α-D-mannose |