CatLogenzyme kinetics catalog

Values CatLog read from this paper

Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase

PMID 15967977doi:10.1073/pnas.0504033102Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 23 records (69 values) from this paper: N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.3.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)Escherichia coliD-3-deoxy-manno-2-octulosonic acid1.213.80.087pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)Escherichia coliL-3-deoxy-manno-2-octulosonic acid1.17.50.15pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)Escherichia coliN-acetyl-D-neuraminic acid10.52.64.04pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)Escherichia coliN-acetyl-L-neuraminic acid2 × 10⁻⁴4.45 × 10⁻⁵pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N1G8Escherichia coliD-3-deoxy-manno-2-octulosonic acid1.512.30.12pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N1G8Escherichia coliL-3-deoxy-manno-2-octulosonic acid1.114.10.08pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N1G8Escherichia coliN-acetyl-D-neuraminic acid10.32.14.98pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N2C5Escherichia coliD-3-deoxy-manno-2-octulosonic acid2.814.50.19pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N2C5Escherichia coliL-3-deoxy-manno-2-octulosonic acid1.99.20.21pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N2C5Escherichia coliN-acetyl-D-neuraminic acid9.73.92.49pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N2C5Escherichia coliN-acetyl-L-neuraminic acid0.0045369.41 × 10⁻⁵pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N3A4Escherichia coliD-3-deoxy-manno-2-octulosonic acid3.23.50.91pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N3A4Escherichia coliL-3-deoxy-manno-2-octulosonic acid2.52.70.92pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N3A4Escherichia coliN-acetyl-D-neuraminic acid2.212.10.18pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N3A4Escherichia coliN-acetyl-L-neuraminic acid0.00681271 × 10⁻⁴pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N4E6Escherichia coliD-3-deoxy-manno-2-octulosonic acid5.16.30.81pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N4E6Escherichia coliL-3-deoxy-manno-2-octulosonic acid4.22.41.75pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N4E6Escherichia coliN-acetyl-D-neuraminic acid1.710.10.17pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N4E6Escherichia coliN-acetyl-L-neuraminic acid0.0236.20.001pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N5B2Escherichia coliD-3-deoxy-manno-2-octulosonic acid8.911.40.78pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N5B2Escherichia coliL-3-deoxy-manno-2-octulosonic acid10.42.83.71pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N5B2Escherichia coliN-acetyl-D-neuraminic acid1.515.30.1pH 7.5, 25 °COpen
N-acetyl-D-neuraminic acid aldolase (sialic acid aldolase)variant N5B2Escherichia coliN-acetyl-L-neuraminic acid0.0233.50.001pH 7.5, 25 °COpen