Values CatLog read from this paper
Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis
PMID 16101288doi:10.1021/bi050493qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (72 values) from this paper: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) | Escherichia coli | 5'-methylthioadenosine | 3 | 8 × 10⁻⁴ | 3.6 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN), 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) | Escherichia coli | S-adenosylhomocysteine | 2.6 | 0.0013 | 2.1 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN), S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F105A | Escherichia coli | 5'-methylthioadenosine | 10.2 | 0.0033 | 3.1 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F105A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F105A | Escherichia coli | S-adenosylhomocysteine | 7.5 | 0.0028 | 2.7 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F105A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F151A | Escherichia coli | 5'-methylthioadenosine | 0.37 | 0.022 | 17 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F151A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F151A | Escherichia coli | S-adenosylhomocysteine | 0.14 | 0.037 | 3.8 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F151A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F207A | Escherichia coli | 5'-methylthioadenosine | 0.13 | 0.0017 | 80 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F207A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant F207A | Escherichia coli | S-adenosylhomocysteine | 0.31 | 0.0055 | 56 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant F207A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant I50A | Escherichia coli | 5'-methylthioadenosine | 3 | 0.0071 | 420 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant I50A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant I50A | Escherichia coli | S-adenosylhomocysteine | 1.8 | 0.0037 | 500 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant I50A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant M173A | Escherichia coli | 5'-methylthioadenosine | 0.72 | 0.034 | 21 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant M173A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant M173A | Escherichia coli | S-adenosylhomocysteine | 0.8 | 0.49 | 1.7 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant M173A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant M9A | Escherichia coli | 5'-methylthioadenosine | 0.63 | 0.0014 | 500 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant M9A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant M9A | Escherichia coli | S-adenosylhomocysteine | 0.37 | 0.001 | 400 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant M9A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant R193A | Escherichia coli | 5'-methylthioadenosine | 1.5 | 0.0032 | 500 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant R193A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant R193A | Escherichia coli | S-adenosylhomocysteine | 1.2 | 0.002 | 600 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant R193A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant S196A | Escherichia coli | 5'-methylthioadenosine | 0.23 | 0.0029 | 81 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant S196A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant S196A | Escherichia coli | S-adenosylhomocysteine | 0.14 | 0.0017 | 84 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant S196A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant S76A | Escherichia coli | 5'-methylthioadenosine | 1.3 | 0.0027 | 500 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant S76A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant S76A | Escherichia coli | S-adenosylhomocysteine | 0.93 | 0.0019 | 500 | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant S76A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant V102A | Escherichia coli | 5'-methylthioadenosine | 10.2 | 0.003 | 3.4 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant V102A, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant V102A | Escherichia coli | S-adenosylhomocysteine | 9.1 | 0.0019 | 4.9 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant V102A, S-adenosylhomocysteine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant Y107F | Escherichia coli | 5'-methylthioadenosine | 2.5 | 0.0022 | 1.2 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant Y107F, 5'-methylthioadenosine |
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN)variant Y107F | Escherichia coli | S-adenosylhomocysteine | 2 | 0.0017 | 1.2 × 10³ | pH 7, 22 °C | Open record: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) variant Y107F, S-adenosylhomocysteine |