Values CatLog read from this paper
Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 1. Crystallography and site-directed mutagenesis of metal binding sites
PMID 1610791doi:10.1021/bi00139a005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (28 values) from this paper: xylose (glucose) isomerase from Actinoplanes missouriensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| xylose (glucose) isomerase | Actinoplanes missouriensis | D-glucose | 24.9 | 290 | 0.086 calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase, D-glucose |
| xylose (glucose) isomerase | Actinoplanes missouriensis | D-xylose | 17.3 | 4.8 | 3.6 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase, D-xylose |
| xylose (glucose) isomerasevariant D255N | Actinoplanes missouriensis | D-xylose | 0.5 | 66 | 0.0076 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant D255N, D-xylose |
| xylose (glucose) isomerasevariant D257E | Actinoplanes missouriensis | D-glucose | 17.7 | 5.8 × 10³ | 0.0031 calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase variant D257E, D-glucose |
| xylose (glucose) isomerasevariant D257E | Actinoplanes missouriensis | D-xylose | 3.4 | 48 | 0.071 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant D257E, D-xylose |
| xylose (glucose) isomerasevariant D257N | Actinoplanes missouriensis | D-glucose | 5 | 300 | 0.017 calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase variant D257N, D-glucose |
| xylose (glucose) isomerasevariant D257N | Actinoplanes missouriensis | D-xylose | 0.9 | 49 | 0.018 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant D257N, D-xylose |
| xylose (glucose) isomerasevariant E181D | Actinoplanes missouriensis | D-glucose | 9.3 | 2.4 × 10³ | 0.0039 calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase variant E181D, D-glucose |
| xylose (glucose) isomerasevariant E181D | Actinoplanes missouriensis | D-xylose | 1.5 | 73 | 0.021 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant E181D, D-xylose |
| xylose (glucose) isomerasevariant E181Q | Actinoplanes missouriensis | D-xylose | 0.1 | 50 | 0.0020 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant E181Q, D-xylose |
| xylose (glucose) isomerasevariant E217S | Actinoplanes missouriensis | D-xylose | 0.08 | — | — | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant E217S, D-xylose |
| xylose (glucose) isomerasevariant H220N | Actinoplanes missouriensis | D-glucose | 0.2 | 500 | 4.0 × 10⁻⁴ calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase variant H220N, D-glucose |
| xylose (glucose) isomerasevariant H220N | Actinoplanes missouriensis | D-xylose | 0.05 | — | — | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant H220N, D-xylose |
| xylose (glucose) isomerasevariant H220Q | Actinoplanes missouriensis | D-glucose | 0.7 | 400 | 0.0018 calc. | pH 7.5, 60 °C | Open record: xylose (glucose) isomerase variant H220Q, D-glucose |
| xylose (glucose) isomerasevariant H220Q | Actinoplanes missouriensis | D-xylose | 0.6 | 16 | 0.037 calc. | pH 7.5, 35 °C | Open record: xylose (glucose) isomerase variant H220Q, D-xylose |