Values CatLog read from this paper
Change of the donor substrate specificity of Clostridium difficile toxin B by site-directed mutagenesis
PMID 16157585doi:10.1074/jbc.m506836200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546), α551 (Clostridium novyi α-toxin enzyme domain, residues 1-551) from Clostridium difficile, Clostridium novyi.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) | Clostridium difficile | uridine 5'-diphosphate-alpha-D-glucose | 0.589 | 0.006 | 97 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546), uridine 5'-diphosphate-alpha-D-glucose |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) | Clostridium difficile | uridine 5'-diphosphate-N-acetyl-D-glucosamine | 0.0031 | 0.96 | 0.0031 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546), uridine 5'-diphosphate-N-acetyl-D-glucosamine |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant I383S | Clostridium difficile | uridine 5'-diphosphate-alpha-D-glucose | 0.5 | 0.008 | 67 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant I383S, uridine 5'-diphosphate-alpha-D-glucose |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant I383S | Clostridium difficile | uridine 5'-diphosphate-N-acetyl-D-glucosamine | 0.117 | 0.061 | 1.92 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant I383S, uridine 5'-diphosphate-N-acetyl-D-glucosamine |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant I383S/Q385A | Clostridium difficile | uridine 5'-diphosphate-alpha-D-glucose | 0.422 | 0.018 | 22.8 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant I383S/Q385A, uridine 5'-diphosphate-alpha-D-glucose |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant I383S/Q385A | Clostridium difficile | uridine 5'-diphosphate-N-acetyl-D-glucosamine | 0.661 | 0.026 | 25.3 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant I383S/Q385A, uridine 5'-diphosphate-N-acetyl-D-glucosamine |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant Q385A | Clostridium difficile | uridine 5'-diphosphate-alpha-D-glucose | 0.45 | 0.008 | 58 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant Q385A, uridine 5'-diphosphate-alpha-D-glucose |
| toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546)variant Q385A | Clostridium difficile | uridine 5'-diphosphate-N-acetyl-D-glucosamine | 0.1428 | 0.052 | 2.78 | pH 7.5, 37 °C | Open record: toxin-B546 (Clostridium difficile toxin B enzyme domain, residues 1-546) variant Q385A, uridine 5'-diphosphate-N-acetyl-D-glucosamine |
| α551 (Clostridium novyi α-toxin enzyme domain, residues 1-551)variant S385I/A387Q | Clostridium novyi | uridine 5'-diphosphate-alpha-D-glucose | 0.39 | 0.004 | 92 | pH 7.5, 37 °C | Open record: α551 (Clostridium novyi α-toxin enzyme domain, residues 1-551) variant S385I/A387Q, uridine 5'-diphosphate-alpha-D-glucose |