CatLogenzyme kinetics catalog

Values CatLog read from this paper

Alpha-isoenzyme of alcohol dehydrogenase from monkey liver. Cloning, expression, mechanism, coenzyme, and substrate specificity

PMID 1618764doi:10.1016/s0021-9258(18)42318-6Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 56 records (98 values) from this paper: alcohol dehydrogenase alpha-isoenzyme (MmADHalpha), liver alcohol dehydrogenase EE isoenzyme (EqADH), monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme) from Macaca mulatta, Equus caballus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 42 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta(R)-2-butanol1.90.424.5 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta(S)-2-butanol1.41.41.0 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta(S)-2-methyl-1-butanol0.890.253.6 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta1-butanol2.20.1912 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta1-hexanol1.60.03250 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta1-pentanol1.80.04540 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta1-propanol1.420.70 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta2-methyl-1-butanol0.770.23.9 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta2-methyl-1-propanol0.621.20.52 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta2-propanol1.63.50.46 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta3-methyl-1-butanol2.10.121 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulatta3-pentanol0.480.222.2 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaacetaldehyde74362.1 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattabenzyl alcohol0.750.03621 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattacyclohexanolalcohol varied at fixed 2 mM NAD+30.024120 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattacyclohexanolinitial velocity study; fitted with SEQUEN3.40.009380 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattacyclohexanone360.35100 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaethanolalcohol varied at fixed 2 mM NAD+1.1150.073 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaethanol1.1140.079 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattamethanol0.00713801.9 × 10⁻⁵ calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaNAD+initial velocity study; fitted with SEQUEN—0.26—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaNAD+cosubstrate ethanol—0.1—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaNAD+——9030 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaNADHinitial velocity study; fitted with SEQUEN—0.008—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)Macaca mulattaNADHcosubstrate acetaldehyde—0.028—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47HMacaca mulattaacetaldehyde240942.6 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47HMacaca mulattaethanol4.41400.031 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47HMacaca mulattaNAD+—2.3—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47HMacaca mulattaNADH—0.13—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47RMacaca mulattaacetaldehyde31161.9 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47RMacaca mulattaethanol0.47100.047 calc.pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47RMacaca mulattaNAD+—0.04—pH 7.3, 30 °COpen
alcohol dehydrogenase alpha-isoenzyme (MmADHalpha)variant G47RMacaca mulattaNADH—0.01—pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus(R)-2-butanol1.7160.11 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus(S)-2-butanol1.24.10.29 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus(S)-2-methyl-1-butanol5.70.3118 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus1-butanol5.20.2719 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus1-hexanol3.80.07650 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus1-pentanol4.30.1529 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus1-propanol8.20.3921 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus2-methyl-1-butanol5.30.5210 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus2-methyl-1-propanol5.30.413 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus2-propanol0.58240.024 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus3-methyl-1-butanol2.80.1519 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballus3-pentanol1.85.50.33 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballusbenzyl alcohol3.60.04678 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballuscyclohexanol6.11.15.5 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballusethanol7.80.89.8 calc.pH 7.3, 30 °COpen
liver alcohol dehydrogenase EE isoenzyme (EqADH)Equus caballusmethanol0.163105.2 × 10⁻⁴ calc.pH 7.3, 30 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulatta1-propanol—0.27—pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulatta2-propanol—3.9—pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulattaacetaldehyde1400.18780 calc.pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulattaethanol4.40.09745 calc.pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulattamethanol—33—pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulattaNAD+—0.007—pH 7.3, 37 °COpen
monkey liver alcohol dehydrogenase (native liver form, similar to human beta-isoenzyme)Macaca mulattaNADH—0.011—pH 7.3, 37 °COpen