Values CatLog read from this paper
Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration
PMID 16204481doi:10.1128/aem.71.10.5728-5734.2005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (73 values) from this paper: phosphite dehydrogenase (PTDH) from Pseudomonas stutzeri.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D (parent) | Pseudomonas stutzeri | NAD+ | 4.37 | 0.066 | 67 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D (parent), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D (parent) | Pseudomonas stutzeri | phosphite | — | 0.057 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D (parent), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/I150F (1-25E8) | Pseudomonas stutzeri | NAD+ | 4.37 | 0.075 | 58 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/I150F (1-25E8), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/I150F (1-25E8) | Pseudomonas stutzeri | phosphite | — | 0.099 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/I150F (1-25E8), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q132R (1-23C7) | Pseudomonas stutzeri | NAD+ | 3.97 | 0.06 | 67 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q132R (1-23C7), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q132R (1-23C7) | Pseudomonas stutzeri | phosphite | — | 0.045 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q132R (1-23C7), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q132R/Q137R/I150F/Q215L/R275Q (5x) | Pseudomonas stutzeri | NAD+ | 2.8 | 0.046 | 62 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q132R/Q137R/I150F/Q215L/R275Q (5x), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q132R/Q137R/I150F/Q215L/R275Q (5x) | Pseudomonas stutzeri | phosphite | — | 0.075 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q132R/Q137R/I150F/Q215L/R275Q (5x), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R (1-1A9) | Pseudomonas stutzeri | NAD+ | 4.75 | 0.066 | 72 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R (1-1A9), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R (1-1A9) | Pseudomonas stutzeri | phosphite | — | 0.048 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R (1-1A9), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q (4x) | Pseudomonas stutzeri | NAD+ | 3.63 | 0.074 | 48 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q (4x), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q (4x) | Pseudomonas stutzeri | phosphite | — | 0.144 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q (4x), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/A319E (2-9B6) | Pseudomonas stutzeri | NAD+ | 3.3 | 0.052 | 63 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/A319E (2-9B6), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/A319E (2-9B6) | Pseudomonas stutzeri | phosphite | — | 0.092 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/A319E (2-9B6), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/D162N/V315A (2-2C10) | Pseudomonas stutzeri | NAD+ | 2.85 | 0.065 | 43 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/D162N/V315A (2-2C10), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/D162N/V315A (2-2C10) | Pseudomonas stutzeri | phosphite | — | 0.155 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/D162N/V315A (2-2C10), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q (2-17C2) | Pseudomonas stutzeri | NAD+ | 2.9 | 0.056 | 52 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q (2-17C2), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q (2-17C2) | Pseudomonas stutzeri | phosphite | — | 0.12 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q (2-17C2), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A (7x) | Pseudomonas stutzeri | NAD+ | 2.9 | 0.066 | 43 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A (7x), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A (7x) | Pseudomonas stutzeri | phosphite | — | 0.096 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A (7x), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V (3-135H2) | Pseudomonas stutzeri | NAD+ | 3.55 | 0.05 | 72 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V (3-135H2), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V (3-135H2) | Pseudomonas stutzeri | phosphite | — | 0.135 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/A325V (3-135H2), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K (3-89D3) | Pseudomonas stutzeri | NAD+ | 3.6 | 0.075 | 48 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K (3-89D3), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K (3-89D3) | Pseudomonas stutzeri | phosphite | — | 0.123 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/E130K (3-89D3), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L (3-110G7) | Pseudomonas stutzeri | NAD+ | 3.6 | 0.071 | 50 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L (3-110G7), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L (3-110G7) | Pseudomonas stutzeri | phosphite | — | 0.156 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/I313L (3-110G7), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R (3-34G8) | Pseudomonas stutzeri | NAD+ | 3.2 | 0.057 | 57 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R (3-34G8), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R (3-34G8) | Pseudomonas stutzeri | phosphite | — | 0.169 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R (3-34G8), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x) | Pseudomonas stutzeri | NAD+ | 3.25 | 0.04 | 82 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x) | Pseudomonas stutzeri | NADP+ | 1.33 | — | — | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x), NADP+ | |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x) | Pseudomonas stutzeri | phosphite | — | 0.046 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/Q132R/V71I/E130K/I313L/A325V (12x), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I (3-57E11) | Pseudomonas stutzeri | NAD+ | 3.5 | 0.063 | 55 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I (3-57E11), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I (3-57E11) | Pseudomonas stutzeri | phosphite | — | 0.105 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q137R/I150F/Q215L/R275Q/L276Q/A319E/V315A/V71I (3-57E11), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q215L (1-11A6) | Pseudomonas stutzeri | NAD+ | 4.63 | 0.064 | 72 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q215L (1-11A6), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/Q215L (1-11A6) | Pseudomonas stutzeri | phosphite | — | 0.058 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/Q215L (1-11A6), phosphite |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/R275Q (1-8D6) | Pseudomonas stutzeri | NAD+ | 4.07 | 0.07 | 58 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/R275Q (1-8D6), NAD+ |
| phosphite dehydrogenase (PTDH)variant D13E/M26I/E175A/E332N/C336D/R275Q (1-8D6) | Pseudomonas stutzeri | phosphite | — | 0.078 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant D13E/M26I/E175A/E332N/C336D/R275Q (1-8D6), phosphite |