Values CatLog read from this paper

Mutagenesis of Glycine 179 modulates both catalytic efficiency and reduced pyridine nucleotide specificity in cytochrome b5 reductase

PMID 16216070doi:10.1021/bi051165tOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (42 values) from this paper: cytochrome b5 reductase (cb5r) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.6.2.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
cytochrome b5 reductase (cb5r)	Rattus norvegicus	cytochrome b5	400	0.013	3.1 × 10⁴ calc.	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r), cytochrome b5
cytochrome b5 reductase (cb5r)	Rattus norvegicus	ferricyanide	—	0.008	—	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r), ferricyanide
cytochrome b5 reductase (cb5r)	Rattus norvegicus	NADH	800	0.006	1.4 × 10⁵	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r), NADH
cytochrome b5 reductase (cb5r)variant G179A	Rattus norvegicus	cytochrome b5	245	0.008	3.1 × 10⁴ calc.	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179A, cytochrome b5
cytochrome b5 reductase (cb5r)variant G179A	Rattus norvegicus	ferricyanide	—	0.008	—	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179A, ferricyanide
cytochrome b5 reductase (cb5r)variant G179A	Rattus norvegicus	NADH	559	0.025	2.3 × 10⁴	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179A, NADH
cytochrome b5 reductase (cb5r)variant G179A	Rattus norvegicus	NADPH	48	1.36	39	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179A, NADPH
cytochrome b5 reductase (cb5r)variant G179P	Rattus norvegicus	cytochrome b5	10	0.001	1.0 × 10⁴ calc.	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179P, cytochrome b5
cytochrome b5 reductase (cb5r)variant G179P	Rattus norvegicus	ferricyanide	—	0.007	—	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179P, ferricyanide
cytochrome b5 reductase (cb5r)variant G179P	Rattus norvegicus	NADH	42	0.595	71	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179P, NADH
cytochrome b5 reductase (cb5r)variant G179P	Rattus norvegicus	NADPH	17	2.317	8.4	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179P, NADPH
cytochrome b5 reductase (cb5r)variant G179T	Rattus norvegicus	cytochrome b5	18	0.043	420 calc.	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179T, cytochrome b5
cytochrome b5 reductase (cb5r)variant G179T	Rattus norvegicus	ferricyanide	—	0.008	—	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179T, ferricyanide
cytochrome b5 reductase (cb5r)variant G179T	Rattus norvegicus	NADH	33	0.662	51	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179T, NADH
cytochrome b5 reductase (cb5r)variant G179T	Rattus norvegicus	NADPH	12	0.507	26	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179T, NADPH
cytochrome b5 reductase (cb5r)variant G179V	Rattus norvegicus	cytochrome b5	17	0.107	160 calc.	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179V, cytochrome b5
cytochrome b5 reductase (cb5r)variant G179V	Rattus norvegicus	ferricyanide	—	0.007	—	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179V, ferricyanide
cytochrome b5 reductase (cb5r)variant G179V	Rattus norvegicus	NADH	12	1.077	11	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179V, NADH
cytochrome b5 reductase (cb5r)variant G179V	Rattus norvegicus	NADPH	8	0.375	22	pH 7, 25 °C	Open record: cytochrome b5 reductase (cb5r) variant G179V, NADPH