Values CatLog read from this paper
Molecular properties and enhancement of thermostability by random mutagenesis of glutamate dehydrogenase from Bacillus subtilis
PMID 16244435doi:10.1271/bbb.69.1861Open in Browse
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CatLog read 11 records (22 values) from this paper: glutamate dehydrogenase (Bs-GluDH) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamate dehydrogenase (Bs-GluDH) | Bacillus subtilis | 2-oxoglutarate | 342 | 0.65 | 535 | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH), 2-oxoglutarate |
| glutamate dehydrogenase (Bs-GluDH) | Bacillus subtilis | ammonium chloride | — | 55.6 | — | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH), ammonium chloride |
| glutamate dehydrogenase (Bs-GluDH) | Bacillus subtilis | L-glutamate | 18.1 | 0.34 | 53.2 | pH 7.7 | Open record: glutamate dehydrogenase (Bs-GluDH), L-glutamate |
| glutamate dehydrogenase (Bs-GluDH) | Bacillus subtilis | NAD+ | — | 0.08 | — | pH 7.7 | Open record: glutamate dehydrogenase (Bs-GluDH), NAD+ |
| glutamate dehydrogenase (Bs-GluDH) | Bacillus subtilis | NADH | — | 0.07 | 4.89 × 10³ | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH), NADH |
| glutamate dehydrogenase (Bs-GluDH)variant E27F | Bacillus subtilis | 2-oxoglutarate | 344 | 0.93 | 270 | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant E27F, 2-oxoglutarate |
| glutamate dehydrogenase (Bs-GluDH)variant E27F | Bacillus subtilis | ammonium chloride | — | 52.3 | — | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant E27F, ammonium chloride |
| glutamate dehydrogenase (Bs-GluDH)variant E27F | Bacillus subtilis | NADH | — | 0.16 | 2.15 × 10³ | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant E27F, NADH |
| glutamate dehydrogenase (Bs-GluDH)variant Q144R | Bacillus subtilis | 2-oxoglutarate | 435 | 1.22 | 356 | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant Q144R, 2-oxoglutarate |
| glutamate dehydrogenase (Bs-GluDH)variant Q144R | Bacillus subtilis | ammonium chloride | — | 56.8 | — | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant Q144R, ammonium chloride |
| glutamate dehydrogenase (Bs-GluDH)variant Q144R | Bacillus subtilis | NADH | — | 0.41 | 1.06 × 10³ | pH 7.3, 37 °C | Open record: glutamate dehydrogenase (Bs-GluDH) variant Q144R, NADH |