CatLogenzyme kinetics catalog

Values CatLog read from this paper

Asp79 makes a large, unfavorable contribution to the stability of RNase Sa

PMID 16288913doi:10.1016/j.jmb.2005.09.091Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (21 values) from this paper: ribonuclease Sa (RNase Sa) from Streptomyces aureofaciens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.6.1.24

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ribonuclease Sa (RNase Sa)Streptomyces aureofacienspolyinosinic acid2200.161.4 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79EStreptomyces aureofacienspolyinosinic acid2200.161.4 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79IStreptomyces aureofacienspolyinosinic acid4100.361.1 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79KStreptomyces aureofacienspolyinosinic acid4300.331.3 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79NStreptomyces aureofacienspolyinosinic acid2800.211.3 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79RStreptomyces aureofacienspolyinosinic acid4600.31.5 × 10³pH 6.5, 25 °COpen
ribonuclease Sa (RNase Sa)variant D79WStreptomyces aureofacienspolyinosinic acid4600.41.2 × 10³pH 6.5, 25 °COpen