Values CatLog read from this paper
Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030
PMID 1629157doi:10.1128/jb.174.15.5013-5020.1992Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (12 values) from this paper: glucose dehydrogenase GlcDH-III, glucose dehydrogenase GlcDH-IV from Bacillus megaterium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glucose dehydrogenase GlcDH-III | Bacillus megaterium | D-glucosecosubstrate NAD | — | 44 | — | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-III, D-glucose |
| glucose dehydrogenase GlcDH-III | Bacillus megaterium | D-glucosesimple Michaelis-Menten fit varying D-glucose at 5.1 mM NADP | — | 135 | — | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-III, D-glucose |
| glucose dehydrogenase GlcDH-III | Bacillus megaterium | NAD+ | 430 | 0.16 | 2.7 × 10³ calc. | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-III, NAD+ |
| glucose dehydrogenase GlcDH-III | Bacillus megaterium | NADP+ | 802 | 18.9 | 42.4 calc. | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-III, NADP+ |
| glucose dehydrogenase GlcDH-IV | Bacillus megaterium | D-glucoseNAD 0.47-2 mM and D-glucose 3.9-98; cosubstrate NAD | — | 9.8 | — | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-IV, D-glucose |
| glucose dehydrogenase GlcDH-IV | Bacillus megaterium | D-glucoseNADP 0.04-5.1 mM and D-glucose 3.9-195; cosubstrate NADP | — | 4.5 | — | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-IV, D-glucose |
| glucose dehydrogenase GlcDH-IV | Bacillus megaterium | NAD+ | 420 | 0.088 | 4.8 × 10³ calc. | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-IV, NAD+ |
| glucose dehydrogenase GlcDH-IV | Bacillus megaterium | NADP+ | 462 | 1.9 | 240 calc. | pH 8, 30 °C | Open record: glucose dehydrogenase GlcDH-IV, NADP+ |