Values CatLog read from this paper
Lys300 plays a major role in the catalytic mechanism of maize polyamine oxidase
PMID 16331971doi:10.1021/bi050983iOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (31 values) from this paper: polyamine oxidase (MPAO) from Zea mays.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| polyamine oxidase (MPAO) | Zea mays | dioxygen | — | 0.083 | — | pH 4, 25 °C | Open record: polyamine oxidase (MPAO), dioxygen |
| polyamine oxidase (MPAO) | Zea mays | spermidinemature protein with C-terminal His6 tag | 50.2 | 0.0021 | 2.39 × 10⁴ | pH 6, 25 °C | Open record: polyamine oxidase (MPAO), spermidine |
| polyamine oxidase (MPAO) | Zea mays | spermidinepurified from maize seedlings (cv. Paolo) | 55.1 | 0.0017 | 3.24 × 10⁴ | pH 6, 25 °C | Open record: polyamine oxidase (MPAO), spermidine |
| polyamine oxidase (MPAO) | Zea mays | spermidine | 31 | 2.12 | 15 calc. | pH 4, 25 °C | Open record: polyamine oxidase (MPAO), spermidine |
| polyamine oxidase (MPAO) | Zea mays | spermidine | 13.4 | — | — | 25 °C | Open record: polyamine oxidase (MPAO), spermidine |
| polyamine oxidase (MPAO) | Zea mays | sperminemature protein with C-terminal His6 tag | 32.9 | 0.0016 | 2.1 × 10⁴ calc. | pH 6, 25 °C | Open record: polyamine oxidase (MPAO), spermine |
| polyamine oxidase (MPAO) | Zea mays | sperminepurified from maize seedlings (cv. Paolo) | 39.3 | 0.0014 | 2.8 × 10⁴ calc. | pH 6, 25 °C | Open record: polyamine oxidase (MPAO), spermine |
| polyamine oxidase (MPAO)variant E170Q | Zea mays | spermidine | 17.3 | 0.0167 | 1 × 10³ | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant E170Q, spermidine |
| polyamine oxidase (MPAO)variant E170Q | Zea mays | spermine | 4.6 | 0.0125 | 370 calc. | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant E170Q, spermine |
| polyamine oxidase (MPAO)variant E62Q | Zea mays | spermidine | 8.3 | 0.0246 | 300 | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant E62Q, spermidine |
| polyamine oxidase (MPAO)variant E62Q | Zea mays | spermine | 2.8 | 0.0147 | 190 calc. | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant E62Q, spermine |
| polyamine oxidase (MPAO)variant K300M | Zea mays | spermidine | 0.0044 | — | — | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant K300M, spermidine |
| polyamine oxidase (MPAO)variant K300M | Zea mays | spermine | 0.0053 | — | — | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant K300M, spermine |
| polyamine oxidase (MPAO)variant Y298F | Zea mays | spermidine | 16.5 | 7 × 10⁻⁴ | 2.36 × 10⁴ | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant Y298F, spermidine |
| polyamine oxidase (MPAO)variant Y298F | Zea mays | spermine | 7.7 | 0.0012 | 6.4 × 10³ calc. | pH 6, 25 °C | Open record: polyamine oxidase (MPAO) variant Y298F, spermine |