Values CatLog read from this paper
Diversity of function in the isocitrate lyase enzyme superfamily: the Dianthus caryophyllus petal death protein cleaves alpha-keto and alpha-hydroxycarboxylic acids
PMID 16342929doi:10.1021/bi051776lOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (47 values) from this paper: petal death protein (PDP), oxaloacetate hydrolase from Dianthus caryophyllus, Botryotinia fuckeliana.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| oxaloacetate hydrolase | Botryotinia fuckeliana | oxaloacetate5 mM MgCl2 | 15.5 | 0.065 | 240 | pH 7.6, 25 °C | Open record: oxaloacetate hydrolase, oxaloacetate |
| oxaloacetate hydrolase | Botryotinia fuckeliana | oxaloacetate0.3 mM MnCl2 | 45.7 | 0.25 | 200 | pH 7.6, 25 °C | Open record: oxaloacetate hydrolase, oxaloacetate |
| petal death protein (PDP) | Dianthus caryophyllus | (2R)-2-ethylmalic acid | 2.47 | 1.1 | 2 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R)-2-ethylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3R:2S,3S)-2-methyl isocitrate | 5.6 × 10⁻⁴ | 0.104 | 0.005 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3R:2S,3S)-2-methyl isocitrate |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2,3-dimethylmalic acidcontinuous coupled assay: LDH/NADH (340 nm) | 0.0337 | 0.026 | 1 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2,3-dimethylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2,3-dimethylmalic acid | 0.028 | 0.029 | 1 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2,3-dimethylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2-ethyl-3-methylmalic acidcontinuous coupled assay: LDH/NADH (340 nm) | 8.4 | 0.53 | 20 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2-ethyl-3-methylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2-ethyl-3-methylmalic acid | 2.18 | 0.45 | 5 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2-ethyl-3-methylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2-isobutyl-3-methylmalic acid | 0.58 | 8 | 0.07 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2-isobutyl-3-methylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-2-propyl-3-methylmalic acid | 0.281 | 0.098 | 3 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-2-propyl-3-methylmalic acid |
| petal death protein (PDP) | Dianthus caryophyllus | (2R,3S)-isocitrate | 1.3 × 10⁻⁴ | 0.042 | 0.003 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (2R,3S)-isocitrate |
| petal death protein (PDP) | Dianthus caryophyllus | (R)-citramalate | 0.21 | 0.29 | 0.7 | pH 7.5, 25 °C | Open record: petal death protein (PDP), (R)-citramalate |
| petal death protein (PDP) | Dianthus caryophyllus | oxaloacetate5 mM MgCl2 | 2.72 | 0.13 | 20 | pH 7.6, 25 °C | Open record: petal death protein (PDP), oxaloacetate |
| petal death protein (PDP) | Dianthus caryophyllus | oxaloacetate0.3 mM MnCl2 | 37 | 0.54 | 70 | pH 7.6, 25 °C | Open record: petal death protein (PDP), oxaloacetate |
| petal death protein (PDP) | Dianthus caryophyllus | threo-(2R,3S:2S,3R)-2-methylisocitrate | 1.4 × 10⁻⁴ | 0.069 | 0.002 | pH 7.5, 25 °C | Open record: petal death protein (PDP), threo-(2R,3S:2S,3R)-2-methylisocitrate |
| petal death protein (PDP)variant D79A | Dianthus caryophyllus | (2R,3S)-2-ethyl-3-methylmalic acid | 0.0023 | 0.6 | 0.0038 calc. | pH 7.5, 25 °C | Open record: petal death protein (PDP) variant D79A, (2R,3S)-2-ethyl-3-methylmalic acid |