Values CatLog read from this paper
Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI
PMID 16464851doi:10.1074/jbc.m513896200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (106 values) from this paper: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) from Escherichia coli, Azoarcus evansii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | 3,4-dihydroxyphenylacetyl-coenzyme A | 18 | 0.051 | 400 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | 3,5-dihydroxyphenylacetyl-coenzyme A | 2.3 | 0.028 | 80 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3,5-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | 3-hydroxyphenylacetyl-coenzyme A | 8.2 | 0.08 | 100 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | 4-hydroxyphenylacetyl-coenzyme A | 11 | 0.055 | 200 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | acetyl-coenzyme A | 0.062 | 1.7 | 0.04 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), acetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | butyryl-coenzyme A | 0.087 | 1.2 | 0.07 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), butyryl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | crotonyl-coenzyme A | 0.0049 | 1.8 | 0.003 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), crotonyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | hexanoyl-coenzyme A | 0.032 | 1.6 | 0.02 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), hexanoyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | isobutyryl-coenzyme A | 0.28 | 1.5 | 0.2 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), isobutyryl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | methylmalonyl-coenzyme A | 0.047 | 1.1 | 0.04 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), methylmalonyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | phenylacetyl-coenzyme A | 3.7 | 0.39 | 10 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), phenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Azoarcus evansii | β-Hydroxybutyryl-CoA | 0.017 | 0.68 | 0.03 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), β-Hydroxybutyryl-CoA |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant D75N | Azoarcus evansii | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.0065 | 0.048 | 0.1 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant D75N, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N60A | Azoarcus evansii | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.012 | 0.0086 | 1 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N60A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N60D | Azoarcus evansii | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.18 | 0.055 | 3 | pH 7.5, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N60D, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 100 | 0.016 | 7 × 10³ | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Escherichia coli | 3,5-dihydroxyphenylacetyl-coenzyme A | 9 | 0.2 | 50 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3,5-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 86 | 0.021 | 4 × 10³ | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 79 | 0.035 | 2 × 10³ | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) | Escherichia coli | phenylacetyl-coenzyme A | 0.41 | 0.0096 | 40 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase), phenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant D16A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.11 | 0.033 | 3 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant D16A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant D16A | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 0.065 | 0.09 | 0.7 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant D16A, 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant D16A | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 0.17 | 0.25 | 0.7 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant D16A, 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant D61A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.0044 | — | — | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant D61A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant E14A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 20 | 0.13 | 200 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant E14A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant E14A | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 15 | 0.08 | 200 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant E14A, 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant E14A | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 19 | 0.09 | 200 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant E14A, 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant H52A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 2.7 | 0.045 | 60 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant H52A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant H52A | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 0.89 | 0.032 | 30 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant H52A, 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant H52A | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 0.26 | 0.028 | 9 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant H52A, 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N15A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 26 | 0.047 | 600 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N15A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N15A | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 30 | 0.034 | 900 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N15A, 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N15A | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 3.2 | 0.018 | 200 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N15A, 4-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N46A | Escherichia coli | 3,4-dihydroxyphenylacetyl-coenzyme A | 0.076 | 0.024 | 3 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N46A, 3,4-dihydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N46A | Escherichia coli | 3-hydroxyphenylacetyl-coenzyme A | 0.81 | 0.23 | 4 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N46A, 3-hydroxyphenylacetyl-coenzyme A |
| PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase)variant N46A | Escherichia coli | 4-hydroxyphenylacetyl-coenzyme A | 0.31 | 0.28 | 1 | pH 8.1, 25 °C | Open record: PaaI (phenylacetate pathway hot dog-fold acyl-CoA thioesterase) variant N46A, 4-hydroxyphenylacetyl-coenzyme A |