Values CatLog read from this paper
Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L1
PMID 16515533doi:10.1042/bj20051645Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: thioesterase I/protease I/phospholipase L1 (TAP) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thioesterase I/protease I/phospholipase L1 (TAP) | Escherichia coli | lauroyl-CoA | 10.13 | 0.146 | 69.44 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP), lauroyl-CoA |
| thioesterase I/protease I/phospholipase L1 (TAP) | Escherichia coli | N-carbobenzoxy-L-tyrosine p-nitrophenyl ester | 88.99 | 0.174 | 512.4 | pH 7.5, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP), N-carbobenzoxy-L-tyrosine p-nitrophenyl ester |
| thioesterase I/protease I/phospholipase L1 (TAP) | Escherichia coli | p-nitrophenyl butyrate | 15.29 | 0.87 | 17.75 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP), p-nitrophenyl butyrate |
| thioesterase I/protease I/phospholipase L1 (TAP)variant D154A | Escherichia coli | lauroyl-CoA | 0.45 | 0.059 | 7.62 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant D154A, lauroyl-CoA |
| thioesterase I/protease I/phospholipase L1 (TAP)variant D154A | Escherichia coli | N-carbobenzoxy-L-tyrosine p-nitrophenyl ester | 6.44 | 0.043 | 149.6 | pH 7.5, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant D154A, N-carbobenzoxy-L-tyrosine p-nitrophenyl ester |
| thioesterase I/protease I/phospholipase L1 (TAP)variant D154A | Escherichia coli | p-nitrophenyl butyrate | 5.98 | 1.88 | 3.18 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant D154A, p-nitrophenyl butyrate |
| thioesterase I/protease I/phospholipase L1 (TAP)variant G44A | Escherichia coli | lauroyl-CoA | 0.77 | 0.075 | 10.19 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant G44A, lauroyl-CoA |
| thioesterase I/protease I/phospholipase L1 (TAP)variant G44A | Escherichia coli | N-carbobenzoxy-L-tyrosine p-nitrophenyl ester | 7.71 | 0.06 | 127.9 | pH 7.5, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant G44A, N-carbobenzoxy-L-tyrosine p-nitrophenyl ester |
| thioesterase I/protease I/phospholipase L1 (TAP)variant G44A | Escherichia coli | p-nitrophenyl butyrate | 3.16 | 1.1 | 2.87 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant G44A, p-nitrophenyl butyrate |
| thioesterase I/protease I/phospholipase L1 (TAP)variant H157A | Escherichia coli | p-nitrophenyl butyrate | 0.0093 | 0.61 | 0.015 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant H157A, p-nitrophenyl butyrate |
| thioesterase I/protease I/phospholipase L1 (TAP)variant N73A | Escherichia coli | lauroyl-CoA | 1.14 | 0.085 | 13.35 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant N73A, lauroyl-CoA |
| thioesterase I/protease I/phospholipase L1 (TAP)variant N73A | Escherichia coli | N-carbobenzoxy-L-tyrosine p-nitrophenyl ester | 7.81 | 0.044 | 178.9 | pH 7.5, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant N73A, N-carbobenzoxy-L-tyrosine p-nitrophenyl ester |
| thioesterase I/protease I/phospholipase L1 (TAP)variant N73A | Escherichia coli | p-nitrophenyl butyrate | 11.99 | 1.66 | 7.22 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant N73A, p-nitrophenyl butyrate |
| thioesterase I/protease I/phospholipase L1 (TAP)variant S10A | Escherichia coli | lauroyl-CoA | 0.057 | 0.127 | 0.45 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant S10A, lauroyl-CoA |
| thioesterase I/protease I/phospholipase L1 (TAP)variant S10A | Escherichia coli | N-carbobenzoxy-L-tyrosine p-nitrophenyl ester | 0.02 | 0.061 | 0.32 | pH 7.5, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant S10A, N-carbobenzoxy-L-tyrosine p-nitrophenyl ester |
| thioesterase I/protease I/phospholipase L1 (TAP)variant S10A | Escherichia coli | p-nitrophenyl butyrate | 0.16 | 1.55 | 0.1 | pH 7, 37 °C | Open record: thioesterase I/protease I/phospholipase L1 (TAP) variant S10A, p-nitrophenyl butyrate |