Values CatLog read from this paper
Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis
PMID 16541109doi:10.1038/sj.emboj.7601026Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (28 values) from this paper: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue from Clostridium perfringens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide0.2 nM enzyme; 1.5-25 µM substrate; 7 min | 10.5 | 0.0029 | 3.6 × 10³ | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminidePUGNAc Ki experiment: steady-state kinetics at 0; 15 | 8 | 0.0026 | 3.1 × 10³ calc. | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue | Clostridium perfringens | p-nitrophenyl-GlcNAc | 0.2 | 0.121 | 1.7 calc. | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue, p-nitrophenyl-GlcNAc | |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant D297N | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 0.0198 | 0.003 | 1 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant D297N, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant D298N | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 0.0013 | 0.004 | 0.3 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant D298N, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant D401A | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 0.0044 | 0.015 | 0.3 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant D401A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant N390A | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 11 | 0.0031 | 3.6 × 10³ | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant N390A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant N396A | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 0.0025 | 0.013 | 0.2 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant N396A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant W490A | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 4 | 0.028 | 100 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant W490A, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |
| NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologuevariant Y335F | Clostridium perfringens | 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | 0.0028 | 0.041 | 0.1 | pH 5.5, 20 °C | Open record: NagJ (CpNagJ), family 84 glycoside hydrolase O-GlcNAcase homologue variant Y335F, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide |