Values CatLog read from this paper
Discrimination of esterase and peptidase activities of acylaminoacyl peptidase from hyperthermophilic Aeropyrum pernix K1 by a single mutation
PMID 16670095doi:10.1074/jbc.m601015200Open in Browse
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CatLog read 14 records (42 values) from this paper: acylaminoacyl peptidase (apAPH) from Aeropyrum pernix.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acylaminoacyl peptidase (apAPH) | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 9.3 | 0.4 | 22.3 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH), N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH) | Aeropyrum pernix | p-nitrophenyl caprylate | 6.6 | 0.0433 | 152.4 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH), p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526A | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 12.6 | 4.3 | 2.9 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526A, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526A | Aeropyrum pernix | p-nitrophenyl caprylate | 9.6 | 0.0416 | 230.7 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526A, p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526E | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 0.5 | 5.8 | 0.09 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526E, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526E | Aeropyrum pernix | p-nitrophenyl caprylate | 8.1 | 0.1146 | 70.7 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526E, p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526I | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 9.2 | 2.1 | 4.4 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526I, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526I | Aeropyrum pernix | p-nitrophenyl caprylate | 20.8 | 0.0401 | 518.7 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526I, p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526K | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 26.9 | 10.5 | 2.6 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526K, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526K | Aeropyrum pernix | p-nitrophenyl caprylate | 9.4 | 0.0382 | 246.1 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526K, p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526L | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 9.6 | 1.3 | 7.4 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526L, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526L | Aeropyrum pernix | p-nitrophenyl caprylate | 28.5 | 0.0382 | 746.1 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526L, p-nitrophenyl caprylate |
| acylaminoacyl peptidase (apAPH)variant R526V | Aeropyrum pernix | N-acetyl-L-leucine p-nitroanilide | 9.7 | 1.7 | 5.7 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526V, N-acetyl-L-leucine p-nitroanilide |
| acylaminoacyl peptidase (apAPH)variant R526V | Aeropyrum pernix | p-nitrophenyl caprylate | 30.9 | 0.0357 | 865.5 | pH 8, 80 °C | Open record: acylaminoacyl peptidase (apAPH) variant R526V, p-nitrophenyl caprylate |