Values CatLog read from this paper
Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase
PMID 16675457doi:10.1074/jbc.m602549200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (60 values) from this paper: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity from Clostridium thermocellum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 30 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity | Clostridium thermocellum | 4-nitrophenyl phosphate | 30.8 | 12 | 2.6 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM NiCl2 | 1.7 | 6.3 | 0.27 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM MnCl2 | 23.2 | 88 | 0.26 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236A | Clostridium thermocellum | 4-nitrophenyl phosphate | 9.58 | 4.9 | 2.0 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236A, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM NiCl2 | 2.53 | 1.6 | 1.6 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM MnCl2 | 43.5 | 12 | 3.6 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236E | Clostridium thermocellum | 4-nitrophenyl phosphate | 1.88 | 6.6 | 0.29 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236E, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236N | Clostridium thermocellum | 4-nitrophenyl phosphate | 38 | 5 | 7.7 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236N, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236N | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM NiCl2 | 9.33 | 2.2 | 4.2 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236N, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D236N | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM MnCl2 | 65.8 | 5 | 13 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D236N, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D392E | Clostridium thermocellum | 4-nitrophenyl phosphate | 3.42 | 1.3 | 2.6 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D392E, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D392E | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 8.8 | 5 | 1.8 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D392E, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant D392N | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 13.77 | 7.4 | 1.9 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant D392N, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H189A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 3.72 | 6.6 | 0.56 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H189A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H189D | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 36 | 3.6 | 10 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H189D, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H189E | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 117.5 | 58 | 2.0 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H189E, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H189Q | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 3.33 | 26 | 0.13 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H189Q, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM NiCl2 | 18 | 3.4 | 5.3 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM MnCl2 | 253.3 | 24 | 11 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264N | Clostridium thermocellum | 4-nitrophenyl phosphate | 147 | 75 | 2.0 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264N, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264N | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 12 | 9.6 | 1.2 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264N, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264Q | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM NiCl2 | 0.92 | 11 | 0.083 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264Q, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H264Q | Clostridium thermocellum | bis(4-nitrophenyl) phosphate0.5 mM MnCl2 | 52.3 | 38 | 1.4 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H264Q, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H376D | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 2.13 | 5 | 0.43 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H376D, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H376N | Clostridium thermocellum | 4-nitrophenyl phosphate | 5.25 | 1 | 5.2 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H376N, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant H376N | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 4.05 | 7.2 | 0.56 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant H376N, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant R237A | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 19.3 | 18 | 1.1 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant R237A, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant R237K | Clostridium thermocellum | 4-nitrophenyl phosphate | 2.5 | 13 | 0.19 calc. | pH 7.5, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant R237K, 4-nitrophenyl phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant R237K | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 3.63 | 12 | 0.30 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant R237K, bis(4-nitrophenyl) phosphate |
| polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activityvariant R237Q | Clostridium thermocellum | bis(4-nitrophenyl) phosphate | 33 | 18 | 1.8 calc. | pH 8, 45 °C | Open record: polynucleotide kinase/phosphatase (CthPnkp), phosphoesterase activity variant R237Q, bis(4-nitrophenyl) phosphate |