Values CatLog read from this paper
Engineering the substrate specificity of porcine kidney D-amino acid oxidase by mutagenesis of the "active-site lid"
PMID 16751595doi:10.1093/jb/mvj094Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 44 records (92 values) from this paper: D-amino acid oxidase (DAO), D-aspartate oxidase (DDO) from Sus scrofa, Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 38 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidase (DAO) | Sus scrofa | D-alanineapparent kcat/Km | 8.5 | 1.1 | 7.8 | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-alanine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-alanine | 6.5 | 0.77 | 8.4 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-alanine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-arginineapparent kcat/Km | 3.2 | 12 | 0.28 | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-arginine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-arginine | 3.5 | 13 | 0.27 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-arginine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-lysine | 0.8 | 10 | 0.080 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-lysine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-methionine | 6.8 | 0.65 | 11 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-methionine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-phenylalanine | 17 | 1.4 | 12 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-phenylalanine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-proline | 10.3 | 0.56 | 18 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-proline |
| D-amino acid oxidase (DAO) | Sus scrofa | D-serine | 3 | 3.3 | 0.91 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-serine |
| D-amino acid oxidase (DAO) | Sus scrofa | D-valine | 2.5 | 0.63 | 4.0 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO), D-valine |
| D-amino acid oxidase (DAO)variant DXO (DAO-DDO hybrid: DAO I215-N225 replaced by R216-G220 of human DDO) | Sus scrofa | D-alanine | 0.88 | 45 | 0.02 | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant DXO (DAO-DDO hybrid: DAO I215-N225 replaced by R216-G220 of human DDO), D-alanine |
| D-amino acid oxidase (DAO)variant DXO (DAO-DDO hybrid: DAO I215-N225 replaced by R216-G220 of human DDO) | Sus scrofa | D-aspartate | 0.5 | 26 | 0.018 | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant DXO (DAO-DDO hybrid: DAO I215-N225 replaced by R216-G220 of human DDO), D-aspartate |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-alanine | 3 | 44 | 0.068 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-alanine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-arginine | 4 | 3 | 1.3 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-arginine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-lysine | 3.3 | 14 | 0.24 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-lysine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-methionine | 10.5 | 0.67 | 16 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-methionine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-phenylalanine | 53 | 1.4 | 38 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-phenylalanine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-proline | 4.3 | 4.1 | 1.1 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-proline |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-serine | 2.2 | — | — | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-serine |
| D-amino acid oxidase (DAO)variant E220D/Y224G | Sus scrofa | D-valine | 1.05 | 3.1 | 0.34 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant E220D/Y224G, D-valine |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-arginine | 4 | 1.7 | 2.4 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-arginine |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-lysine | 5 | 20 | 0.25 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-lysine |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-methionine | 2.5 | 0.61 | 4.1 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-methionine |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-phenylalanine | 3.5 | 0.57 | 6.1 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-phenylalanine |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-proline | 3.5 | 22 | 0.16 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-proline |
| D-amino acid oxidase (DAO)variant G222D/Y224G | Sus scrofa | D-valine | 0.25 | 5.1 | 0.049 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant G222D/Y224G, D-valine |
| D-amino acid oxidase (DAO)variant I223D/Y224G | Sus scrofa | D-arginine | 1.8 | 5.9 | 0.31 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant I223D/Y224G, D-arginine |
| D-amino acid oxidase (DAO)variant I223D/Y224G | Sus scrofa | D-lysine | 3 | 20 | 0.15 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant I223D/Y224G, D-lysine |
| D-amino acid oxidase (DAO)variant I223D/Y224G | Sus scrofa | D-methionine | 1.3 | 0.77 | 1.7 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant I223D/Y224G, D-methionine |
| D-amino acid oxidase (DAO)variant I223D/Y224G | Sus scrofa | D-phenylalanine | 3 | 1.3 | 2.3 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant I223D/Y224G, D-phenylalanine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-alanine | 1.42 | 46 | 0.031 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-alanine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-arginine | 7.5 | 3 | 2.5 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-arginine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-lysine | 5 | 20 | 0.25 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-lysine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-methionine | 9 | 0.67 | 13 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-methionine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-phenylalanine | 13 | 0.77 | 17 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-phenylalanine |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-proline | 3.8 | 7.7 | 0.50 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-proline |
| D-amino acid oxidase (DAO)variant R221D/Y224G | Sus scrofa | D-valine | 0.75 | 3.7 | 0.20 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant R221D/Y224G, D-valine |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-arginine | 2 | 5.1 | 0.39 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-arginine |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-lysine | 2.3 | 18 | 0.13 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-lysine |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-methionine | 1.38 | 0.77 | 1.8 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-methionine |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-phenylalanine | 3.2 | 1 | 3.2 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-phenylalanine |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-proline | 1.7 | 29 | 0.057 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-proline |
| D-amino acid oxidase (DAO)variant Y224D | Sus scrofa | D-valine | 0.43 | 22 | 0.020 calc. | pH 8.3, 25 °C | Open record: D-amino acid oxidase (DAO) variant Y224D, D-valine |
| D-aspartate oxidase (DDO) | Homo sapiens | D-aspartate | 47 | 2.2 | 22 | pH 8.3, 25 °C | Open record: D-aspartate oxidase (DDO), D-aspartate |