Values CatLog read from this paper
Identification of a novel NADH-specific aldo-keto reductase using sequence and structural homologies
PMID 16813561doi:10.1042/bj20060660Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (73 values) from this paper: aldo-keto reductase AKR11B2 (ec2), aldo-keto reductase AKR3F1 (tm1), aldo-keto reductase AKR3C2 (sp2) and 3 more from Escherichia coli, Thermotoga maritima, Schizosaccharomyces pombe and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 36 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
EC 1.1.1.2, EC 1.1.1.190, EC 1.1.1.191, EC 1.1.1.346
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aldo-keto reductase AKR11B2 (ec2) | Escherichia coli | DL-glyceraldehydecosubstrate NADH | 2 | 363 | 0.0055 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2), DL-glyceraldehyde |
| aldo-keto reductase AKR11B2 (ec2) | Escherichia coli | DL-glyceraldehydeDL-glyceraldehyde varied at saturating NADPH | — | — | 8.6 × 10⁻⁶ | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2), DL-glyceraldehyde |
| aldo-keto reductase AKR11B2 (ec2) | Escherichia coli | methylglyoxal | 15.7 | 1.81 | 8.67 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2), methylglyoxal |
| aldo-keto reductase AKR11B2 (ec2) | Escherichia coli | NADH | 5.6 | 0.052 | 110 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2), NADH |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | DL-glyceraldehydecosubstrate NADH | 23.6 | 1.2 × 10³ | 0.020 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, DL-glyceraldehyde |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | DL-glyceraldehydecosubstrate NADPH | 1.5 | 218 | 0.0069 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, DL-glyceraldehyde |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | methylglyoxalcosubstrate NADH | 40.1 | 5.2 | 7.7 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, methylglyoxal |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | methylglyoxalcosubstrate NADPH | 5.1 | 5.51 | 0.93 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, methylglyoxal |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | NADH | 11.4 | 0.083 | 140 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, NADH |
| aldo-keto reductase AKR11B2 (ec2)variant D232A | Escherichia coli | NADPH | 1.95 | 0.11 | 18 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232A, NADPH |
| aldo-keto reductase AKR11B2 (ec2)variant D232E | Escherichia coli | methylglyoxalcosubstrate NADH | 100.1 | 7.8 | 13 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232E, methylglyoxal |
| aldo-keto reductase AKR11B2 (ec2)variant D232E | Escherichia coli | methylglyoxalcosubstrate NADPH | 4.09 | 0.13 | 31 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232E, methylglyoxal |
| aldo-keto reductase AKR11B2 (ec2)variant D232E | Escherichia coli | NADH | 15.7 | 0.0282 | 557 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232E, NADH |
| aldo-keto reductase AKR11B2 (ec2)variant D232E | Escherichia coli | NADPH | 4.1 | 0.1358 | 30 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR11B2 (ec2) variant D232E, NADPH |
| aldo-keto reductase AKR2E3 (dm1) | Drosophila melanogaster | DL-glyceraldehyde | 40 | 9.5 | 4.2 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR2E3 (dm1), DL-glyceraldehyde |
| aldo-keto reductase AKR2E3 (dm1) | Drosophila melanogaster | NADPH | 25.1 | 0.0426 | 589 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR2E3 (dm1), NADPH |
| aldo-keto reductase AKR3C2 (sp2) | Schizosaccharomyces pombe | DL-glyceraldehyde | 2.5 | 552 | 0.0045 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2), DL-glyceraldehyde |
| aldo-keto reductase AKR3C2 (sp2) | Schizosaccharomyces pombe | Isatincosubstrate NADH | 1.4 | 0.017 | 82 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2), Isatin |
| aldo-keto reductase AKR3C2 (sp2) | Schizosaccharomyces pombe | Isatincosubstrate NADPH | 33 | 0.017 | 1.9 × 10³ calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2), Isatin |
| aldo-keto reductase AKR3C2 (sp2) | Schizosaccharomyces pombe | NADH | 4.2 | 0.0533 | 79 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2), NADH |
| aldo-keto reductase AKR3C2 (sp2) | Schizosaccharomyces pombe | NADPH | 11.8 | 0.034 | 350 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2), NADPH |
| aldo-keto reductase AKR3C2 (sp2)variant D202S | Schizosaccharomyces pombe | Isatin | 91.7 | 0.43 | 210 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2) variant D202S, Isatin |
| aldo-keto reductase AKR3C2 (sp2)variant D202S | Schizosaccharomyces pombe | NADH | 56.4 | 0.378 | 149 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3C2 (sp2) variant D202S, NADH |
| aldo-keto reductase AKR3F1 (tm1) | Thermotoga maritima | DL-glyceraldehydecosubstrate NADH | 4 | 52 | 0.077 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1), DL-glyceraldehyde |
| aldo-keto reductase AKR3F1 (tm1) | Thermotoga maritima | DL-glyceraldehydecosubstrate NADPH | 4.8 | 137 | 0.035 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1), DL-glyceraldehyde |
| aldo-keto reductase AKR3F1 (tm1) | Thermotoga maritima | NADH | 3.3 | 0.026 | 130 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1), NADH |
| aldo-keto reductase AKR3F1 (tm1) | Thermotoga maritima | NADPH | 0.75 | 0.0056 | 130 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1), NADPH |
| aldo-keto reductase AKR3F1 (tm1)variant E208S | Thermotoga maritima | DL-glyceraldehyde | 111.8 | 210.8 | 0.5304 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1) variant E208S, DL-glyceraldehyde |
| aldo-keto reductase AKR3F1 (tm1)variant E208S | Thermotoga maritima | Isatincosubstrate NADH | 30.1 | 0.61 | 49 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1) variant E208S, Isatin |
| aldo-keto reductase AKR3F1 (tm1)variant E208S | Thermotoga maritima | Isatincosubstrate NADPH | 17.3 | 0.047 | 370 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1) variant E208S, Isatin |
| aldo-keto reductase AKR3F1 (tm1)variant E208S | Thermotoga maritima | NADH | 11.8 | 0.0378 | 312 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1) variant E208S, NADH |
| aldo-keto reductase AKR3F1 (tm1)variant E208S | Thermotoga maritima | NADPH | 17.2 | 0.0301 | 571 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F1 (tm1) variant E208S, NADPH |
| aldo-keto reductase AKR3F2 (ec1) | Escherichia coli | 4-nitrobenzaldehyde | 74.9 | 1.78 | 42.1 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F2 (ec1), 4-nitrobenzaldehyde |
| aldo-keto reductase AKR3F2 (ec1) | Escherichia coli | NADPH | 50.8 | 0.1 | 510 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F2 (ec1), NADPH |
| aldo-keto reductase AKR3F3 (sm1) | Sinorhizobium meliloti | DL-glyceraldehyde | 2 | 256 | 0.0078 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F3 (sm1), DL-glyceraldehyde |
| aldo-keto reductase AKR3F3 (sm1) | Sinorhizobium meliloti | Isatin | 6.6 | 7 | 0.94 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F3 (sm1), Isatin |
| aldo-keto reductase AKR3F3 (sm1) | Sinorhizobium meliloti | NADPH | 16.7 | 0.0407 | 410 calc. | pH 7, 25 °C | Open record: aldo-keto reductase AKR3F3 (sm1), NADPH |