CatLogenzyme kinetics catalog

Values CatLog read from this paper

Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01

PMID 16844437doi:10.1016/j.bbapap.2006.05.010Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 33 records (99 values) from this paper: CumD (meta-cleavage product hydrolase) from Pseudomonas fluorescens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.7.1.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
CumD (meta-cleavage product hydrolase)Pseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.0370.002217pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)Pseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate23.10.00782.96 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)Pseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate19.50.00892.16 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)Pseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate42.40.0113.82 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129VPseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.010.011pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129VPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate250.00564.5 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129VPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate810.00988.3 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129VPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate750.00184.2 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/I199VPseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.010.0171pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/I199VPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate4.50.00351.3 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/I199VPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate190.00484 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/I199VPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate350.0057 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/V227IPseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.0020.00580.3pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/V227IPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate0.730.0170pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/V227IPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate290.00555.3 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant A129V/V227IPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate240.00241 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant F104MPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate6.40.0041.6 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant F104MPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate6.50.00391.7 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant F104MPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate15.40.00275.8 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant I199VPseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.024.5 × 10⁻⁴44pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant I199VPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate290.00142.1 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant I199VPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate380.00351.1 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant I199VPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate370.00231.6 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34APseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate6.70.0051.4 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34APseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate0.30.0025110pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34APseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate1.30.0031420pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34GPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate8.18 × 10⁻⁴1.1 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34GPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate1.50.0026560pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant S34GPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate8.40.00422 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant V227IPseudomonas fluorescens2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate0.020.00494pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant V227IPseudomonas fluorescens2-hydroxy-6-oxo-7-methylocta-2,4-dienoate200.00494.1 × 10³pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant V227IPseudomonas fluorescens2-hydroxy-6-oxohepta-2,4-dienoate520.00281.9 × 10⁴pH 7.5, 25 °COpen
CumD (meta-cleavage product hydrolase)variant V227IPseudomonas fluorescens2-hydroxy-6-oxoocta-2,4-dienoate780.0023.9 × 10⁴pH 7.5, 25 °COpen