CatLogenzyme kinetics catalog

Values CatLog read from this paper

Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 A resolution: construction of a NADH-accepting mutant and its application in rare sugar…

PMID 16906761doi:10.1021/bi052589qOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (50 values) from this paper: 1,5-anhydro-D-fructose reductase (AFR) from Sinorhizobium morelense.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.292

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
1,5-anhydro-D-fructose reductase (AFR)Sinorhizobium morelense1,5-anhydro-D-fructose2168.326.02pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)Sinorhizobium morelense1,5-anhydro-D-fructoserecombinant; expressed in E. coli BL21(DE3) from pET-24a(+)1456.422.65pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)Sinorhizobium morelenseNADPH—0.1—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)Sinorhizobium morelenseNADPHrecombinant; expressed in E. coli BL21(DE3) from pET-24a(+)—0.06—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13GSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADPH; cosubstrate NADPH4058.547.65pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13GSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADH; cosubstrate NADH12.411.11.11pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13GSinorhizobium morelenseNADH—1.1—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13GSinorhizobium morelenseNADPH—0.02—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S10GSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADPH; cosubstrate NADPH3697.151.97pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S10GSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADH; cosubstrate NADH5.5390.14pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S10GSinorhizobium morelenseNADH—1.2—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S10GSinorhizobium morelenseNADPH—0.38—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S33DSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADPH; cosubstrate NADPH6.320.20.31pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S33DSinorhizobium morelense1,5-anhydro-D-fructosecofactor NADH; cosubstrate NADH13.53.24.21pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S33DSinorhizobium morelenseNADH—1.1—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant A13G/S33DSinorhizobium morelenseNADPH—1—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant D176ASinorhizobium morelense1,5-anhydro-D-fructose1.3490.026pH 7.5Open
1,5-anhydro-D-fructose reductase (AFR)variant G206ISinorhizobium morelense1,5-anhydro-D-fructose1568.318.79pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant G206ISinorhizobium morelenseNADPH—0.06—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant H180ASinorhizobium morelense1,5-anhydro-D-fructose3.78.90.42pH 8Open
1,5-anhydro-D-fructose reductase (AFR)variant K94GSinorhizobium morelense1,5-anhydro-D-fructose4.222.50.19pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant K94GSinorhizobium morelenseNADPH—0.2—pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant S10GSinorhizobium morelense1,5-anhydro-D-fructose1193.534pH 6.5Open
1,5-anhydro-D-fructose reductase (AFR)variant S10GSinorhizobium morelenseNADPH—0.27—pH 6.5Open