Values CatLog read from this paper
Mutation of Lys242 allows Delta3-Delta2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity
PMID 16952422doi:10.1016/j.bbagen.2006.08.001Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (26 values) from this paper: Δ3-Δ2-enoyl-CoA isomerase (ECI), enoyl-CoA hydratase (ECH) from Rattus norvegicus, Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| enoyl-CoA hydratase (ECH) | Bos taurus | trans-2-hexenoyl-CoA | 180 | 0.0067 | 2.7 × 10⁴ calc. | pH 8 | Open record: enoyl-CoA hydratase (ECH), trans-2-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI) | Rattus norvegicus | cis-3-hexenoyl-CoA | 252 | 0.079 | 3.2 × 10³ calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI), cis-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI) | Rattus norvegicus | trans-3-hexenoyl-CoA | 160 | 0.048 | 3.3 × 10³ calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI), trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant D149E | Rattus norvegicus | trans-3-hexenoyl-CoA | 70 | 0.08 | 880 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant D149E, trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242C | Rattus norvegicus | cis-3-hexenoyl-CoA | 1.29 | 0.013 | 99 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242C, cis-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242C | Rattus norvegicus | trans-2-hexenoyl-CoA | 1 | 0.31 | 3.2 calc. | pH 8 | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242C, trans-2-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242C | Rattus norvegicus | trans-3-hexenoyl-CoA | 11 | 0.12 | 92 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242C, trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242D | Rattus norvegicus | trans-2-hexenoyl-CoA | 0.013 | 0.0026 | 5.0 calc. | pH 8 | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242D, trans-2-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242D | Rattus norvegicus | trans-3-hexenoyl-CoA | 0.06 | 0.03 | 2.0 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242D, trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242F | Rattus norvegicus | trans-3-hexenoyl-CoA | 0.14 | 0.1 | 1.4 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242F, trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242R | Rattus norvegicus | trans-3-hexenoyl-CoA | 50 | 0.089 | 560 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242R, trans-3-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242T | Rattus norvegicus | trans-2-hexenoyl-CoA | 0.021 | 6.2 × 10⁻⁴ | 34 calc. | pH 8 | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242T, trans-2-hexenoyl-CoA |
| Δ3-Δ2-enoyl-CoA isomerase (ECI)variant K242T | Rattus norvegicus | trans-3-hexenoyl-CoA | 0.19 | 0.076 | 2.5 calc. | pH 7.5, 25 °C | Open record: Δ3-Δ2-enoyl-CoA isomerase (ECI) variant K242T, trans-3-hexenoyl-CoA |