Values CatLog read from this paper
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily
PMID 16981709doi:10.1021/bi061208lOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (6 values) from this paper: phosphonopyruvate hydrolase (PPH) from Variovorax sp. Pal2.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphonopyruvate hydrolase (PPH) | Variovorax sp. Pal2 | phosphoenolpyruvate | 2 × 10⁻⁴ | — | — | pH 7.5, 25 °C | Open record: phosphonopyruvate hydrolase (PPH), phosphoenolpyruvate |
| phosphonopyruvate hydrolase (PPH) | Variovorax sp. Pal2 | phosphonopyruvate5 mM MgCl2 (fixed, saturating); P-pyr varied (0.5-5 Km) | 105 | 0.0195 | 5.38 × 10³ calc. | pH 7.5, 25 °C | Open record: phosphonopyruvate hydrolase (PPH), phosphonopyruvate |
| phosphonopyruvate hydrolase (PPH) | Variovorax sp. Pal2 | phosphonopyruvateMg2+ and P-pyr concentrations both varied | — | 0.005 | — | pH 7.5, 25 °C | Open record: phosphonopyruvate hydrolase (PPH), phosphonopyruvate |
| phosphonopyruvate hydrolase (PPH)variant R188A | Variovorax sp. Pal2 | phosphonopyruvate | 121 | 0.082 | 1.5 × 10³ calc. | pH 7.5, 25 °C | Open record: phosphonopyruvate hydrolase (PPH) variant R188A, phosphonopyruvate |