Values CatLog read from this paper
A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression
PMID 17049749doi:10.1016/j.bbagen.2006.08.024Open in Browse
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CatLog read 13 records (13 values) from this paper: D-2-hydroxyacid dehydrogenase (D2-HDH) from Haloferax mediterranei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 2-oxobutanoateNADPH 0.15 mM fixed; cosubstrate NADPH | — | 13.45 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 2-oxobutanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 2-oxobutanoateNADH 0.3 mM fixed; cosubstrate NADH | — | 106 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 2-oxobutanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 3-methyl-2-oxopentanoateNADPH 0.15 mM fixed; cosubstrate NADPH | — | 1.31 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 3-methyl-2-oxopentanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 3-methyl-2-oxopentanoateNADH 0.3 mM fixed; cosubstrate NADH | — | 11.9 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 3-methyl-2-oxopentanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 4-methyl-2-oxopentanoateNADPH 0.15 mM fixed; cosubstrate NADPH | — | 3.77 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 4-methyl-2-oxopentanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | 4-methyl-2-oxopentanoateNADH 0.3 mM fixed; cosubstrate NADH | — | 13.9 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), 4-methyl-2-oxopentanoate |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADH2-ketoisoleucine 25 mM fixed; cosubstrate 2-ketoisoleucine | — | 0.33 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADH2-ketoisocaproate 25 mM fixed; cosubstrate 2-ketoisocaproate | — | 0.233 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADH2-ketobutyrate 25 mM fixed; cosubstrate 2-ketobutyrate | — | 0.5 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADPH2-ketoisoleucine 25 mM fixed; cosubstrate 2-ketoisoleucine | — | 0.046 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADPH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADPH2-ketoisocaproate 25 mM fixed; cosubstrate 2-ketoisocaproate | — | 0.031 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADPH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | NADPH2-ketobutyrate 25 mM fixed; cosubstrate 2-ketobutyrate | — | 0.054 | — | pH 8.5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), NADPH |
| D-2-hydroxyacid dehydrogenase (D2-HDH) | Haloferax mediterranei | pyruvate | — | 21.96 | — | pH 5, 40 °C | Open record: D-2-hydroxyacid dehydrogenase (D2-HDH), pyruvate |