CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular architecture of the glucose 1-phosphate site in ADP-glucose pyrophosphorylases

PMID 17079236doi:10.1074/jbc.m607088200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 41 records (41 values) from this paper: ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.27

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)Escherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA370——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)Escherichia colialpha-D-glucose 1-phosphatethermal stability experiment315.8——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239AEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA32.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment0.5——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239EEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA347.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239EEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment304.3——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239NEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA169——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239NEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 3152——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D239NEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 390.3——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276AEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA0.36——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment0.307——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276EEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA112.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276EEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment89.4——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276NEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA0.37——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant D276NEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment0.3——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194AEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA15.43——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment13——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194DEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA92.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194DEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment75——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194QEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA80.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant E194QEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment66.3——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240AEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA171.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 3111.6——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 356.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240MEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA487——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240MEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 3254.4——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant F240MEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment; Table 3207——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212AEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA371.2——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212AEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment335.7——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212TEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA179——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212TEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment107——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212VEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA22.4——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212VEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment18——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212YEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA1.6——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant S212YEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment1.1——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant W274AEscherichia colialpha-D-glucose 1-phosphate384——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant W274FEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA266——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant W274FEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment133.1——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant W274LEscherichia colialpha-D-glucose 1-phosphate247.5——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant Y216FEscherichia colialpha-D-glucose 1-phosphateAssay B; 50 mM Hepes pH 8.0; saturating ATP; 0.2 mg/ml BSA29——pH 8, 37 °COpen
ADP-glucose pyrophosphorylase (glucose-1-phosphate adenylyltransferase)variant Y216FEscherichia colialpha-D-glucose 1-phosphatethermal stability experiment24.7——pH 8, 37 °COpen