Values CatLog read from this paper
Dual binding sites for translocation catalysis by Escherichia coli glutathionylspermidine synthetase
PMID 17124497doi:10.1038/sj.emboj.7601440Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (30 values) from this paper: glutathionylspermidine synthetase/amidase (GspS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutathionylspermidine synthetase/amidase (GspS) | Escherichia coli | glutathione | 4.35 | 0.218 | 20.0 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS), glutathione | |
| glutathionylspermidine synthetase/amidase (GspS) | Escherichia coli | spermidine | — | 0.076 | — | Open record: glutathionylspermidine synthetase/amidase (GspS), spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant C338A | Escherichia coli | glutathione | 4.28 | 2.098 | 2.04 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant C338A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant C338A | Escherichia coli | spermidine | — | 0.406 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant C338A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant E391A | Escherichia coli | glutathione | 0.43 | 0.407 | 1.1 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant E391A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant E391A | Escherichia coli | spermidine | — | 4.522 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant E391A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant E392A | Escherichia coli | glutathione | 0.75 | 7.298 | 0.10 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant E392A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant E392A | Escherichia coli | spermidine | — | 1.008 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant E392A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant K607A | Escherichia coli | glutathione | 4.55 | 0.251 | 18.1 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant K607A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant K607A | Escherichia coli | spermidine | — | 0.153 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant K607A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant R538A | Escherichia coli | glutathione | 1.01 | 1.324 | 0.763 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant R538A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant R538A | Escherichia coli | spermidine | — | 0.182 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant R538A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant R598A | Escherichia coli | glutathione | 0.29 | 0.0224 | 13 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant R598A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant R598A | Escherichia coli | spermidine | — | 0.665 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant R598A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant S335A | Escherichia coli | glutathione | 3.31 | 0.794 | 4.17 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant S335A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant S335A | Escherichia coli | spermidine | — | 0.126 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant S335A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant S337A | Escherichia coli | glutathione | 2.49 | 0.224 | 11.1 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant S337A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant S337A | Escherichia coli | spermidine | — | 0.076 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant S337A, spermidine | |
| glutathionylspermidine synthetase/amidase (GspS)variant T441A | Escherichia coli | glutathione | 0.25 | 0.691 | 0.36 calc. | Open record: glutathionylspermidine synthetase/amidase (GspS) variant T441A, glutathione | |
| glutathionylspermidine synthetase/amidase (GspS)variant T441A | Escherichia coli | spermidine | — | 1.61 | — | Open record: glutathionylspermidine synthetase/amidase (GspS) variant T441A, spermidine |