Values CatLog read from this paper
Mechanistic characterization of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa
PMID 17128965doi:10.1021/bi061673iOpen in Browse
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CatLog read 10 records (24 values) from this paper: N-formimino-L-glutamate iminohydrolase (HutF) from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N-formimino-L-glutamate iminohydrolase (HutF) | Pseudomonas aeruginosa | N-formimino-L-glutamateZn2+-reconstituted apoenzyme (1.2 Zn2+/subunit) | 13.2 | 0.22 | 60 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF), N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF) | Pseudomonas aeruginosa | N-formimino-L-glutamateNi2+-reconstituted apoenzyme (0.81 Ni2+/subunit) | 21.3 | 2.7 | 8 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF), N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF) | Pseudomonas aeruginosa | N-formimino-L-glutamateCd2+-reconstituted apoenzyme (1.2 Cd2+/subunit) | 31 | 2.1 | 15 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF), N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF) | Pseudomonas aeruginosa | N-formimino-L-glutamateCu2+-reconstituted apoenzyme (1.7 Cu2+/subunit) | 8.5 | 1.2 | 7.3 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF), N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant E235A | Pseudomonas aeruginosa | N-formimino-L-glutamate | 0.1 | 2.2 | 0.047 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant E235A, N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant E235D | Pseudomonas aeruginosa | N-formimino-L-glutamate | 0.005 | 0.66 | 0.0079 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant E235D, N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant E235Q | Pseudomonas aeruginosa | N-formimino-L-glutamate | 0.12 | 19 | 0.0063 | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant E235Q, N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant H269A | Pseudomonas aeruginosa | N-formimino-L-glutamate | — | — | 1.6 × 10⁻⁴ | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant H269A, N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant H269C | Pseudomonas aeruginosa | N-formimino-L-glutamate | — | — | 7.3 × 10⁻⁴ | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant H269C, N-formimino-L-glutamate |
| N-formimino-L-glutamate iminohydrolase (HutF)variant H269N | Pseudomonas aeruginosa | N-formimino-L-glutamate | — | — | 1.8 × 10⁻⁴ | pH 8, 30 °C | Open record: N-formimino-L-glutamate iminohydrolase (HutF) variant H269N, N-formimino-L-glutamate |