Values CatLog read from this paper
Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity
PMID 17145127doi:10.1016/j.biochi.2006.10.017Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (72 values) from this paper: D-amino acid oxidase (RgDAAO) from Rhodotorula gracilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 36 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidase (RgDAAO) | Rhodotorula gracilis | D-arginine | 20 | 18 | 1.1 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO), D-arginine |
| D-amino acid oxidase (RgDAAO)variant M213E | Rhodotorula gracilis | cephalosporin C | 7.3 | 28 | 0.26 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant M213E, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant M213E | Rhodotorula gracilis | D-alanine | 6.9 | 40 | 0.17 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant M213E, D-alanine |
| D-amino acid oxidase (RgDAAO)variant M213E | Rhodotorula gracilis | D-arginine | 3.2 | 1.3 | 2.5 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant M213E, D-arginine |
| D-amino acid oxidase (RgDAAO)variant M213E | Rhodotorula gracilis | D-aspartate | 3.2 | 53 | 0.060 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant M213E, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant M213E | Rhodotorula gracilis | D-glutamate | 3.7 | 150 | 0.025 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant M213E, D-glutamate |
| D-amino acid oxidase (RgDAAO)variant Q339E | Rhodotorula gracilis | cephalosporin C | 24 | 3.8 | 6.3 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339E, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Q339E | Rhodotorula gracilis | D-alanine | 3.7 | 0.4 | 9.2 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339E, D-alanine |
| D-amino acid oxidase (RgDAAO)variant Q339E | Rhodotorula gracilis | D-arginine | 6.5 | 5.5 | 1.2 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339E, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Q339E | Rhodotorula gracilis | D-aspartate | 1 | 61 | 0.016 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339E, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Q339N | Rhodotorula gracilis | cephalosporin C | 4.4 | 1.7 | 2.6 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339N, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Q339N | Rhodotorula gracilis | D-alanine | 0.79 | 1.3 | 0.61 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339N, D-alanine |
| D-amino acid oxidase (RgDAAO)variant Q339N | Rhodotorula gracilis | D-aspartate | 0.37 | 98 | 0.0038 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Q339N, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant S335G | Rhodotorula gracilis | cephalosporin C | 50 | 2.8 | 18 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335G, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant S335G | Rhodotorula gracilis | D-arginine | 8.6 | 6.7 | 1.3 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335G, D-arginine |
| D-amino acid oxidase (RgDAAO)variant S335H | Rhodotorula gracilis | cephalosporin C | 26 | 4.3 | 6.0 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335H, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant S335H | Rhodotorula gracilis | D-alanine | 75 | 1.1 | 68 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335H, D-alanine |
| D-amino acid oxidase (RgDAAO)variant S335H | Rhodotorula gracilis | D-arginine | 2.1 | 2.5 | 0.84 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335H, D-arginine |
| D-amino acid oxidase (RgDAAO)variant S335H | Rhodotorula gracilis | D-aspartate | 0.09 | 5.2 | 0.017 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335H, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant S335R | Rhodotorula gracilis | cephalosporin C | 7 | 2.5 | 2.8 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335R, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant S335R | Rhodotorula gracilis | D-alanine | 9.2 | 0.6 | 15 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335R, D-alanine |
| D-amino acid oxidase (RgDAAO)variant S335R | Rhodotorula gracilis | D-arginine | 3.6 | 13 | 0.28 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335R, D-arginine |
| D-amino acid oxidase (RgDAAO)variant S335R | Rhodotorula gracilis | D-aspartate | 0.67 | 15 | 0.045 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant S335R, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Y223F | Rhodotorula gracilis | D-arginine | 7.3 | 29 | 0.25 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y223F, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Y223F | Rhodotorula gracilis | D-aspartate | 2.5 | 150 | 0.017 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y223F, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Y223S | Rhodotorula gracilis | cephalosporin C | 1.3 | 1 | 1.3 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y223S, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Y223S | Rhodotorula gracilis | D-arginine | 0.43 | 23 | 0.019 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y223S, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Y223S | Rhodotorula gracilis | D-aspartate | 0.9 | 33 | 0.027 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y223S, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-arginine | 11 | 5.5 | 2.0 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Y238F | Rhodotorula gracilis | D-aspartate | 0.26 | 15 | 0.017 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238F, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Y238R | Rhodotorula gracilis | cephalosporin C | 24 | 1 | 24 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238R, cephalosporin C |
| D-amino acid oxidase (RgDAAO)variant Y238R | Rhodotorula gracilis | D-alanine | 15 | 0.5 | 30 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238R, D-alanine |
| D-amino acid oxidase (RgDAAO)variant Y238R | Rhodotorula gracilis | D-arginine | 2.6 | 9.2 | 0.28 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238R, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Y238R | Rhodotorula gracilis | D-aspartate | 6 | 72 | 0.083 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238R, D-aspartate |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-arginine | 14 | 9.7 | 1.4 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-arginine |
| D-amino acid oxidase (RgDAAO)variant Y238S | Rhodotorula gracilis | D-aspartate | 0.56 | 17 | 0.033 calc. | pH 8.5, 25 °C | Open record: D-amino acid oxidase (RgDAAO) variant Y238S, D-aspartate |