Values CatLog read from this paper
Tyrosine83 is essential for the activity of E. coli galactoside transacetylase
PMID 17208523doi:10.1016/j.bbapap.2006.11.013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (29 values) from this paper: galactoside transacetylase (LacA) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| galactoside transacetylase (LacA) | Escherichia coli | 4-nitrophenyl beta-D-galactopyranosideMichaelis-Menten fit | 11.1 | 27.2 | 0.411 | 25 °C | Open record: galactoside transacetylase (LacA), 4-nitrophenyl beta-D-galactopyranoside |
| galactoside transacetylase (LacA) | Escherichia coli | 4-nitrophenyl beta-D-galactopyranosidesingle initial-velocity estimate at 4 mM acceptor (Fig. 3) | — | — | 0.201 | 25 °C | Open record: galactoside transacetylase (LacA), 4-nitrophenyl beta-D-galactopyranoside |
| galactoside transacetylase (LacA) | Escherichia coli | 4-nitrophenyl beta-D-glucopyranoside | — | — | 0.055 | 25 °C | Open record: galactoside transacetylase (LacA), 4-nitrophenyl beta-D-glucopyranoside |
| galactoside transacetylase (LacA) | Escherichia coli | 4-nitrophenyl cellobioside | — | — | 0.015 | 25 °C | Open record: galactoside transacetylase (LacA), 4-nitrophenyl cellobioside |
| galactoside transacetylase (LacA) | Escherichia coli | acetyl-CoA | 11.5 | 0.203 | 56.7 calc. | 25 °C | Open record: galactoside transacetylase (LacA), acetyl-CoA |
| galactoside transacetylase (LacA) | Escherichia coli | isopropyl beta-D-1-thiogalactopyranoside | 122 | 629 | 0.194 | 25 °C | Open record: galactoside transacetylase (LacA), isopropyl beta-D-1-thiogalactopyranoside |
| galactoside transacetylase (LacA)variant Y83F | Escherichia coli | 4-nitrophenyl beta-D-galactopyranoside | 4.9 | 29.5 | 0.166 | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83F, 4-nitrophenyl beta-D-galactopyranoside |
| galactoside transacetylase (LacA)variant Y83F | Escherichia coli | isopropyl beta-D-1-thiogalactopyranoside | 168 | 2.72 × 10³ | 0.062 | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83F, isopropyl beta-D-1-thiogalactopyranoside |
| galactoside transacetylase (LacA)variant Y83L | Escherichia coli | 4-nitrophenyl beta-D-galactopyranoside | 0.006 | 24.1 | 2.5 × 10⁻⁴ | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83L, 4-nitrophenyl beta-D-galactopyranoside |
| galactoside transacetylase (LacA)variant Y83L | Escherichia coli | isopropyl beta-D-1-thiogalactopyranoside | 0.31 | 2.44 × 10³ | 1.3 × 10⁻⁴ | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83L, isopropyl beta-D-1-thiogalactopyranoside |
| galactoside transacetylase (LacA)variant Y83V | Escherichia coli | 4-nitrophenyl beta-D-galactopyranoside | 0.022 | 97 | 2.3 × 10⁻⁴ | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83V, 4-nitrophenyl beta-D-galactopyranoside |
| galactoside transacetylase (LacA)variant Y83V | Escherichia coli | isopropyl beta-D-1-thiogalactopyranoside | 0.175 | 1.15 × 10³ | 1.5 × 10⁻⁴ | 25 °C | Open record: galactoside transacetylase (LacA) variant Y83V, isopropyl beta-D-1-thiogalactopyranoside |