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Phosphoramidate pronucleotides: a comparison of the phosphoramidase substrate specificity of human and Escherichia coli histidine triad nucleotide binding proteins

PMID 17217311doi:10.1021/mp060070yOpen in Browse

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CatLog read 40 records (120 values) from this paper: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase from Escherichia coli, Homo sapiens.

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Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli2(R)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester10.04520pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, 2(R)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli2(R)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.370.02117pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, 2(R)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli2(S)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester1.10.04328pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, 2(S)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli2(S)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.50.03216pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, 2(S)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-adenosyl ester0.520.0638pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-adenosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-guanosyl ester0.560.0678pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-guanosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-adenosyl ester0.070.7790.09pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-adenosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-guanosyl ester0.0110.1370.08pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-guanosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-adenosyl ester0.020.3610.05pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-adenosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-guanosyl ester0.010.190.05pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-guanosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-adenosyl ester1.280.09414pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-adenosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-guanosyl ester0.520.0648pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-guanosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(1-beta-arabinofuranosyl)adenine ester1.50.0630pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(1-beta-arabinofuranosyl)adenine ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(7-benzylguanosyl) ester0.150.0562.6pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(7-benzylguanosyl) ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-adenosyl ester4.50.0052870pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-adenosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-cytidyl ester0.490.0320pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-cytidyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-guanosyl ester40.006700pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-guanosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-inosyl ester4.20.014310pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-inosyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-thymidyl ester0.020.0460.4pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-thymidyl ester
Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidaseEscherichia coli[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-uridyl ester2.40.04270pH 7.2, 25 °COpen record: Escherichia coli histidine triad nucleotide binding protein (echinT, hinT), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-uridyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens2(R)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.270.004170pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, 2(R)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens2(R)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.260.003380pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, 2(R)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens2(S)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.0120.0410.23pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, 2(S)-[adenosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens2(S)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester0.00710.0310.23pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, 2(S)-[guanosyl-5'-(phosphorylamino)]-3-(3-indolyl)propionic acid methyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-adenosyl ester0.020.0440.5pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-adenosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-guanosyl ester0.0240.0650.37pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-(methylcarbamoyl)ethyl]phosphoramidic acid 5'-guanosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-adenosyl ester0.030.0490.6pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-adenosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-guanosyl ester0.0120.1620.07pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Rp)-thiophosphoramidic acid 5'-guanosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-adenosyl ester0.0110.2160.05pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-adenosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-guanosyl ester0.0020.0520.04pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]-(Sp)-thiophosphoramidic acid 5'-guanosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-adenosyl ester0.450.059.2pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-adenosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-guanosyl ester0.230.0584pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1(S)-carbamoylethyl]phosphoramidic acid 5'-guanosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(1-beta-arabinofuranosyl)adenine ester1.10.0011.1 × 10³pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(1-beta-arabinofuranosyl)adenine ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(7-benzylguanosyl) ester0.680.01450pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-(7-benzylguanosyl) ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-adenosyl ester2.11.3 × 10⁻⁴1.5 × 10⁴pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-adenosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-cytidyl ester1.20.0023600pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-cytidyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-guanosyl ester2.32.1 × 10⁻⁴1.1 × 10⁴pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-guanosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-inosyl ester2.67.1 × 10⁻⁴3.7 × 10³pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-inosyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-thymidyl ester0.10.0323pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-thymidyl ester
human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidaseHomo sapiens[2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-uridyl ester2.50.00221.2 × 10³pH 7.2, 25 °COpen record: human histidine triad nucleotide binding protein 1 (hHint1), nucleoside phosphoramidase, [2-(3-indolyl)-1-ethyl]phosphoramidic acid 5'-uridyl ester