Values CatLog read from this paper
Directed evolution of the alpha-L-fucosidase from Thermotoga maritima into an alpha-L-transfucosidase
PMID 17240986doi:10.1021/bi061444wOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 25 records (73 values) from this paper: alpha-L-fucosidase (TmalphaFuc) from Thermotoga maritima.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alpha-L-fucosidase (TmalphaFuc) | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 5.1 | 0.02 | 260 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc), 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc) | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 35 | 0.079 | 440 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc), 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc) | Thermotoga maritima | 5-bromo-4-chloro-3-indolyl alpha-L-fucopyranoside | — | 1.4 | — | Open record: alpha-L-fucosidase (TmalphaFuc), 5-bromo-4-chloro-3-indolyl alpha-L-fucopyranoside | |
| alpha-L-fucosidase (TmalphaFuc)variant G226S/T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 0.48 | 0.09 | 5.3 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant G226S/T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant G226S/T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 6.4 | 0.3 | 21 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant G226S/T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant G226S/Y237H/T264A/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 2.7 | 0.08 | 33 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant G226S/Y237H/T264A/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant G226S/Y237H/T264A/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 39 | 0.1 | 390 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant G226S/Y237H/T264A/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 20 | 0.1 | 200 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 65 | 0.06 | 1.1 × 10³ | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A/Y267F | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 4.3 | 0.09 | 47 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A/Y267F, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A/Y267F | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 56 | 0.14 | 400 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A/Y267F, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 0.73 | 0.14 | 5.2 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant M210V/Y237H/T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 20 | 0.25 | 80 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant M210V/Y237H/T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant P196L/Y237H/Y267F | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 15 | 0.09 | 170 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant P196L/Y237H/Y267F, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant P196L/Y237H/Y267F | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 164 | 0.1 | 1.6 × 10³ | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant P196L/Y237H/Y267F, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant R147G/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 15 | 0.13 | 120 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant R147G/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant R147G/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 198 | 0.28 | 710 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant R147G/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 15 | 0.065 | 230 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 169 | 0.07 | 2.5 × 10³ | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 3.6 | 0.19 | 19 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 50 | 0.25 | 200 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 0.5 | 0.06 | 8.3 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant T264A/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 8.2 | 0.31 | 27 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant T264A/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant Y237H/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 14.8 | 0.29 | 51 | pH 5.5, 40 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant Y237H/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |
| alpha-L-fucosidase (TmalphaFuc)variant Y237H/Y267F/L322P | Thermotoga maritima | 4-nitrophenyl alpha-L-fucopyranoside | 96 | 0.23 | 420 | pH 5, 70 °C | Open record: alpha-L-fucosidase (TmalphaFuc) variant Y237H/Y267F/L322P, 4-nitrophenyl alpha-L-fucopyranoside |