Values CatLog read from this paper
Kinetic characterization of the oxidation of esculetin by polyphenol oxidase and peroxidase
PMID 17284853doi:10.1271/bbb.60431Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (12 values) from this paper: horseradish peroxidase (POD), polyphenol oxidase (mushroom tyrosinase, PPO) from horseradish, mushroom.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| horseradish peroxidase (POD) | horseradish | esculetin | 890 | 2 | 450 calc. | pH 4.5, 25 °C | Open record: horseradish peroxidase (POD), esculetin |
| horseradish peroxidase (POD) | horseradish | esculetin | 871 | 1.4 | 620 calc. | pH 7, 25 °C | Open record: horseradish peroxidase (POD), esculetin |
| horseradish peroxidase (POD) | horseradish | hydrogen peroxide | — | 0.052 | — | pH 4.5, 25 °C | Open record: horseradish peroxidase (POD), hydrogen peroxide |
| horseradish peroxidase (POD) | horseradish | hydrogen peroxide | — | 0.0052 | — | pH 7, 25 °C | Open record: horseradish peroxidase (POD), hydrogen peroxide |
| polyphenol oxidase (mushroom tyrosinase, PPO) | mushroom | esculetin | 551 | 0.9 | 610 calc. | pH 4.5, 25 °C | Open record: polyphenol oxidase (mushroom tyrosinase, PPO), esculetin |
| polyphenol oxidase (mushroom tyrosinase, PPO) | mushroom | esculetin | 928 | 0.63 | 1.5 × 10³ calc. | pH 7, 25 °C | Open record: polyphenol oxidase (mushroom tyrosinase, PPO), esculetin |
| polyphenol oxidase (mushroom tyrosinase, PPO) | mushroom | oxygen | — | 0.041 | — | pH 4.5, 25 °C | Open record: polyphenol oxidase (mushroom tyrosinase, PPO), oxygen |
| polyphenol oxidase (mushroom tyrosinase, PPO) | mushroom | oxygen | — | 0.04 | — | pH 7, 25 °C | Open record: polyphenol oxidase (mushroom tyrosinase, PPO), oxygen |