Values CatLog read from this paper
Cloning and high-level production of a chitinase from Chromobacterium sp. and the role of conserved or nonconserved residues on its catalytic activity
PMID 17294188doi:10.1007/s00253-006-0614-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (14 values) from this paper: Chi54 (chitinase) from Chromobacterium sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Chi54 (chitinase) | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 18 | 0.036 | 500 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase), 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant I270L | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 13 | 0.036 | 360 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant I270L, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant I270V | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 9 | 0.035 | 260 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant I270V, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant N401V | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 20 | 0.042 | 480 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant N401V, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant S497E | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 7 | 0.035 | 200 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant S497E, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant T218S | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 24 | 0.034 | 710 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant T218S, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |
| Chi54 (chitinase)variant V493M | Chromobacterium sp. | 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside | 9 | 0.036 | 250 calc. | pH 7, 37 °C | Open record: Chi54 (chitinase) variant V493M, 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside |