Values CatLog read from this paper
The role of Asp-295 in the catalytic mechanism of Leuconostoc mesenteroides sucrose phosphorylase probed with site-directed mutagenesis
PMID 17350620doi:10.1016/j.febslet.2007.02.060Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (26 values) from this paper: sucrose phosphorylase (LmSPase) from Leuconostoc mesenteroides.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| sucrose phosphorylase (LmSPase) | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphate | — | — | 2.9 | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase), alpha-D-glucose 1-phosphate |
| sucrose phosphorylase (LmSPase) | Leuconostoc mesenteroides | alpha-D-mannose 1-phosphate | — | — | 1.7 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase), alpha-D-mannose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | alpha-D-glucopyranosyl 2-beta-D-fructofuranoside | — | — | 5.3 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, alpha-D-glucopyranosyl 2-beta-D-fructofuranoside |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphatearsenolysis of alphaG1P (glucose release) | — | — | 5.2 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, alpha-D-glucose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphatesucrose synthesis (phosphate release) | 0.0124 | — | 9.2 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, alpha-D-glucose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | alpha-D-mannose 1-phosphate | — | — | 7.4 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, alpha-D-mannose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | arsenate | 0.0065 | — | 6.7 × 10⁻⁴ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, arsenate |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | D-fructose | 0.0014 | — | 4.6 × 10⁻⁴ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, D-fructose |
| sucrose phosphorylase (LmSPase)variant D295E | Leuconostoc mesenteroides | phosphate | 0.0136 | — | 0.00889 | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295E, phosphate |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | alpha-D-glucopyranosyl 2-beta-D-fructofuranoside | 0.0199 | — | 0.00138 | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, alpha-D-glucopyranosyl 2-beta-D-fructofuranoside |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphatearsenolysis of alphaG1P (glucose release) | 0.0141 | — | 2.56 × 10⁻⁴ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, alpha-D-glucose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphatesucrose synthesis (phosphate release) | 0.0159 | — | 7.22 × 10⁻⁴ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, alpha-D-glucose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | alpha-D-mannose 1-phosphate | — | — | 3.3 × 10⁻⁵ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, alpha-D-mannose 1-phosphate |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | arsenate | 0.0252 | — | 0.00256 | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, arsenate |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | D-fructose | 0.0206 | — | 3.9 × 10⁻⁴ | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, D-fructose |
| sucrose phosphorylase (LmSPase)variant D295N | Leuconostoc mesenteroides | phosphate | 0.0238 | — | 0.00205 | pH 7, 30 °C | Open record: sucrose phosphorylase (LmSPase) variant D295N, phosphate |