Values CatLog read from this paper
Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from "Chelatobacter" strain ATCC 29600
PMID 1735711doi:10.1128/jb.174.4.1179-1188.1992Open in Browse
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CatLog read 4 records (4 values) from this paper: nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B) from "Chelatobacter" strain ATCC 29600.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B) | "Chelatobacter" strain ATCC 29600 | flavin mononucleotide | — | 0.0013 | — | pH 8.5, 25 °C | Open record: nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B), flavin mononucleotide |
| nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B) | "Chelatobacter" strain ATCC 29600 | magnesium nitrilotriacetate (equimolar MgCl2 and NTA) | — | 0.5 | — | pH 8.5, 25 °C | Open record: nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B), magnesium nitrilotriacetate (equimolar MgCl2 and NTA) |
| nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B) | "Chelatobacter" strain ATCC 29600 | NADH | — | 0.35 | — | 25 °C | Open record: nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B), NADH |
| nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B) | "Chelatobacter" strain ATCC 29600 | nitrilotriacetate | — | 0.5 | — | pH 8.5, 25 °C | Open record: nitrilotriacetate monooxygenase (NTA-Mo; reconstituted from component A and component B), nitrilotriacetate |