Values CatLog read from this paper
A UDP-glucose:isoflavone 7-O-glucosyltransferase from the roots of soybean (glycine max) seedlings. Purification, gene cloning, phylogenetics, and an implication for an alternative strategy of…
PMID 17565994doi:10.1074/jbc.m702651200Open in Browse
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CatLog read 20 records (50 values) from this paper: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) from Glycine max.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) | Glycine max | genisteinpurified from roots of G. max seedlings | 0.74 | 0.0036 | 210 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT), genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) | Glycine max | genisteinfull-length recombinant; N-terminal His6-tagged (pET-15b) | 0.76 | 0.0027 | 280 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT), genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) | Glycine max | UDP-glucosepurified from roots of G. max seedlings | — | 0.19 | 3.9 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT), UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) | Glycine max | UDP-glucosefull-length recombinant; N-terminal His6-tagged (pET-15b) | — | 0.13 | 5.8 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT), UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant D125A | Glycine max | genistein | 0.66 | 0.06 | 11 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant D125A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant D125A | Glycine max | UDP-glucose | — | 0.045 | 15 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant D125A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E376A | Glycine max | genistein | 0.63 | 0.0045 | 140 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E376A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E376A | Glycine max | UDP-glucose | — | 2.1 | 0.3 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E376A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E392A | Glycine max | genistein | 4 × 10⁻⁴ | 0.011 | 0.036 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E392A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E392A | Glycine max | UDP-glucose | — | 0.19 | 0.0021 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E392A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E392D | Glycine max | genistein | 3 | 7.7 × 10⁻⁴ | 3.9 × 10³ | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E392D, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E392D | Glycine max | UDP-glucose | — | 0.024 | 130 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E392D, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E456A | Glycine max | genistein | 0.41 | 0.0026 | 160 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E456A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant E456A | Glycine max | UDP-glucose | — | 0.082 | 5 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant E456A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H15A | Glycine max | genistein | 0.13 | 0.016 | 8 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H15A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H15A | Glycine max | UDP-glucose | — | 0.027 | 4.8 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H15A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H359A | Glycine max | genistein | 0.59 | 0.0027 | 220 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H359A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H359A | Glycine max | UDP-glucose | — | 0.072 | 8.2 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H359A, UDP-glucose |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H368A | Glycine max | genistein | 2 | 0.0049 | 410 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H368A, genistein |
| UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT)variant H368A | Glycine max | UDP-glucose | — | 0.081 | 25 | pH 8.5, 30 °C | Open record: UDP-glucose:isoflavone 7-O-glucosyltransferase (GmIF7GT) variant H368A, UDP-glucose |