Values CatLog read from this paper
N-Acetyl-D-glucosamine-6-phosphate deacetylase: substrate activation via a single divalent metal ion
PMID 17567047doi:10.1021/bi700543xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (87 values) from this paper: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-galactosamine 6-phosphate | 154 | 1.24 | 120 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-galactosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateZn-reconstituted apo-NagA (0.9 Zn per subunit) | 102 | 0.08 | 1.3 × 10³ | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateCd-reconstituted apo-NagA (0.8 Cd per subunit) | 163 | 0.2 | 820 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateCo-reconstituted apo-NagA (1.0 Co per subunit) | 177 | 0.15 | 1.2 × 10³ | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateNi-reconstituted apo-NagA (0.8 Ni per subunit) | 41 | 0.64 | 64 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateMn-reconstituted apo-NagA (0.7 Mn per subunit) | 92 | 0.1 | 920 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateFe-reconstituted apo-NagA (0.5 Fe per subunit) | 58 | 0.23 | 250 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateZn-NagA; solvent H2O | 78 | 0.14 | 570 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateZn-NagA; solvent D2O; pD 7.5 | 69 | 0.12 | 560 | 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-phosphateinitial NagA preparation (no Zn supplement in growth medium) | 35 | — | — | 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-sulfateZn-substituted NagA (Zn-NagA) | 64 | 4.9 | 13 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-sulfate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-acetyl-D-glucosamine 6-sulfateCd-substituted NagA (Cd-NagA) | 23 | 11 | 2.1 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-acetyl-D-glucosamine 6-sulfate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-formyl-D-glucosamine 6-phosphate | 22 | 0.29 | 76 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-formyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-thioacetyl-D-glucosamine 6-phosphateZn-substituted NagA (Zn-NagA) | 10 | 0.24 | 42 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-thioacetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-thioacetyl-D-glucosamine 6-phosphateCd-substituted NagA (Cd-NagA) | 128 | 0.2 | 640 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-thioacetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-thioacetyl-D-glucosamine 6-phosphateMn-substituted NagA (Mn-NagA) | 11 | 0.23 | 48 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-thioacetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) | Escherichia coli | N-trifluoroacetyl-D-glucosamine 6-phosphate | 2.61 × 10³ | 0.4 | 6.5 × 10³ | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA), N-trifluoroacetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant E131A | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 1.9 | 0.15 | 13 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant E131A, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant E131Q | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 0.7 | 3.6 | 1.9 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant E131Q, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant H143N | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 0.43 | 2.1 | 0.2 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant H143N, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant H143Q | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 2.4 | 0.33 | 7.3 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant H143Q, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant H251N | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 6.8 | 2.7 | 2.5 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant H251N, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant K139M | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 49 | 2 | 25 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant K139M, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant K139M | Escherichia coli | N-acetyl-D-glucosamine 6-sulfate | 60 | 9.7 | 6.2 calc. | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant K139M, N-acetyl-D-glucosamine 6-sulfate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant N61A | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 24 | 0.8 | 30 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant N61A, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant N61H | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 2.6 | 1.7 | 1.5 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant N61H, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant Q59A | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 10 | 0.08 | 130 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant Q59A, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant Q59H | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 32 | 0.31 | 100 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant Q59H, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant Q59H/N61H | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 1.2 | 5.4 | 0.22 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant Q59H/N61H, N-acetyl-D-glucosamine 6-phosphate |
| N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA)variant Y223F | Escherichia coli | N-acetyl-D-glucosamine 6-phosphate | 167 | 0.76 | 220 | pH 7.5, 30 °C | Open record: N-acetyl-D-glucosamine-6-phosphate deacetylase (NagA) variant Y223F, N-acetyl-D-glucosamine 6-phosphate |