Values CatLog read from this paper

Glycine oxidase from Bacillus subtilis: role of histidine 244 and methionine 261

PMID 17573175doi:10.1016/j.biochi.2007.04.019Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (81 values) from this paper: glycine oxidase from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.4.3.19

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
glycine oxidase	Bacillus subtilis	D-alanine	0.92	490	0.002	pH 8.5, 25 °C	Open record: glycine oxidase, D-alanine
glycine oxidase	Bacillus subtilis	D-proline	0.5	17	0.029	pH 8.5, 25 °C	Open record: glycine oxidase, D-proline
glycine oxidase	Bacillus subtilis	glycine	0.6	0.7	0.86	pH 8.5, 25 °C	Open record: glycine oxidase, glycine
glycine oxidase	Bacillus subtilis	N-methylglycine	0.6	0.7	0.86	pH 8.5, 25 °C	Open record: glycine oxidase, N-methylglycine
glycine oxidasevariant H244F	Bacillus subtilis	D-alanine	1.5	530	0.003	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, D-alanine
glycine oxidasevariant H244F	Bacillus subtilis	D-proline	1.35	26	0.052	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, D-proline
glycine oxidasevariant H244F	Bacillus subtilis	dioxygen	—	0.3	—	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, dioxygen
glycine oxidasevariant H244F	Bacillus subtilis	glycine	0.78	0.6	1.3	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, glycine
glycine oxidasevariant H244F	Bacillus subtilis	N-methylglycinesteady-state; 50 mM disodium pyrophosphate buffer pH 8.5	1.08	1.4	0.77	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, N-methylglycine
glycine oxidasevariant H244F	Bacillus subtilis	N-methylglycine75 mM disodium pyrophosphate buffer pH 8.5	5.5	8.4	0.65 calc.	pH 8.5, 25 °C	Open record: glycine oxidase variant H244F, N-methylglycine
glycine oxidasevariant H244N	Bacillus subtilis	D-alanine	0.75	420	0.002	pH 8.5, 25 °C	Open record: glycine oxidase variant H244N, D-alanine
glycine oxidasevariant H244N	Bacillus subtilis	D-proline	0.3	26	0.012	pH 8.5, 25 °C	Open record: glycine oxidase variant H244N, D-proline
glycine oxidasevariant H244N	Bacillus subtilis	glycine	1.3	1.9	0.68	pH 8.5, 25 °C	Open record: glycine oxidase variant H244N, glycine
glycine oxidasevariant H244N	Bacillus subtilis	N-methylglycine	1.33	2.1	0.63	pH 8.5, 25 °C	Open record: glycine oxidase variant H244N, N-methylglycine
glycine oxidasevariant H244Q	Bacillus subtilis	D-alanine	1.08	350	0.003	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, D-alanine
glycine oxidasevariant H244Q	Bacillus subtilis	D-proline	1.23	27	0.046	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, D-proline
glycine oxidasevariant H244Q	Bacillus subtilis	dioxygen	—	0.27	—	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, dioxygen
glycine oxidasevariant H244Q	Bacillus subtilis	glycine	1.2	0.8	1.5	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, glycine
glycine oxidasevariant H244Q	Bacillus subtilis	N-methylglycinesteady-state; 50 mM disodium pyrophosphate buffer pH 8.5	1.12	2.8	0.4	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, N-methylglycine
glycine oxidasevariant H244Q	Bacillus subtilis	N-methylglycine75 mM disodium pyrophosphate buffer pH 8.5	5.5	1.7	3.2 calc.	pH 8.5, 25 °C	Open record: glycine oxidase variant H244Q, N-methylglycine
glycine oxidasevariant M261H	Bacillus subtilis	D-proline	0.62	280	0.002	pH 8.5, 25 °C	Open record: glycine oxidase variant M261H, D-proline
glycine oxidasevariant M261H	Bacillus subtilis	dioxygen	—	0.23	—	pH 8.5, 25 °C	Open record: glycine oxidase variant M261H, dioxygen
glycine oxidasevariant M261H	Bacillus subtilis	glycinesteady-state; 50 mM disodium pyrophosphate buffer pH 8.5	0.55	28	0.02	pH 8.5, 25 °C	Open record: glycine oxidase variant M261H, glycine
glycine oxidasevariant M261H	Bacillus subtilis	glycine75 mM disodium pyrophosphate buffer pH 8.5	2.1	45	0.047 calc.	pH 8.5, 25 °C	Open record: glycine oxidase variant M261H, glycine
glycine oxidasevariant M261H	Bacillus subtilis	N-methylglycine	0.73	11	0.066	pH 8.5, 25 °C	Open record: glycine oxidase variant M261H, N-methylglycine
glycine oxidasevariant M261Y	Bacillus subtilis	D-alanine	0.47	410	0.001	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, D-alanine
glycine oxidasevariant M261Y	Bacillus subtilis	D-proline	0.38	285	0.001	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, D-proline
glycine oxidasevariant M261Y	Bacillus subtilis	dioxygen	—	0.19	—	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, dioxygen
glycine oxidasevariant M261Y	Bacillus subtilis	glycinesteady-state; 50 mM disodium pyrophosphate buffer pH 8.5	0.3	18	0.017	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, glycine
glycine oxidasevariant M261Y	Bacillus subtilis	glycine75 mM disodium pyrophosphate buffer pH 8.5	1.2	80	0.015 calc.	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, glycine
glycine oxidasevariant M261Y	Bacillus subtilis	N-methylglycine	0.47	11	0.043	pH 8.5, 25 °C	Open record: glycine oxidase variant M261Y, N-methylglycine