Values CatLog read from this paper
Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin
PMID 1764912doi:10.1016/0305-0491(91)90050-nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (70 values) from this paper: alpha-chymotrypsin, chymotrypsin A, chymotrypsin B from Gadus morhua, Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) Me2SO | 137 | 0.26 | 527 | pH 8.1, 25 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine ethyl ester |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) methanol | 100 | 0.27 | 370 | pH 8.1, 25 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine ethyl ester |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 25% (v/v) methanol | 25 | 0.52 | 48 | pH 8.1, 25 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine ethyl ester |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine ethyl ester | 142 | 0.24 | 591 | pH 7.8, 35 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine ethyl ester |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine ethyl ester | 43 | 0.27 | 159 | pH 7.8, 10 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine ethyl ester |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine p-nitroanilide | 0.41 | 0.21 | 1.9 | pH 7.8, 35 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine p-nitroanilide |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine p-nitroanilide | 0.3 | 0.14 | 2.2 | pH 7.8, 25 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine p-nitroanilide |
| alpha-chymotrypsin | Bos taurus | N-benzoyl-L-tyrosine p-nitroanilide | 0.05 | 0.11 | 0.4 | pH 7.8, 10 °C | Open record: alpha-chymotrypsin, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) Me2SO | 436 | 0.19 | 2295 | pH 8.1, 25 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) methanol | 207 | 0.14 | 1479 | pH 8.1, 25 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 25% (v/v) methanol | 80 | 0.51 | 157 | pH 8.1, 25 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine ethyl ester | 329 | 0.17 | 1935 | pH 7.8, 35 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine ethyl ester | 117 | 0.12 | 975 | pH 7.8, 10 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.82 | — | 5.9 | pH 7.8, 35 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.41 | 0.08 | 5.1 | pH 7.8, 25 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin A | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.1 | 0.09 | 1.1 calc. | pH 7.8, 10 °C | Open record: chymotrypsin A, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) Me2SO | 441 | 0.36 | 1225 | pH 8.1, 25 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 10% (v/v) methanol | 214 | 0.21 | 1019 | pH 8.1, 25 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine ethyl esterBzTyrOEt dissolved in 25% (v/v) methanol | 41 | 0.51 | 80 | pH 8.1, 25 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine ethyl ester | 276 | 0.1 | 2.76 × 10³ | pH 7.8, 35 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine ethyl ester | 120 | 0.2 | 600 | pH 7.8, 10 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine ethyl ester |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.72 | 0.11 | 7.2 | pH 7.8, 35 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.42 | 0.08 | 5.3 | pH 7.8, 25 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine p-nitroanilide |
| chymotrypsin B | Gadus morhua | N-benzoyl-L-tyrosine p-nitroanilide | 0.1 | 0.11 | 0.9 | pH 7.8, 10 °C | Open record: chymotrypsin B, N-benzoyl-L-tyrosine p-nitroanilide |