Values CatLog read from this paper

Role of the amino acid invariants in the active site of MurG as evaluated by site-directed mutagenesis

PMID 17692452doi:10.1016/j.biochi.2007.06.011Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (45 values) from this paper: MurG from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.227

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
MurG	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0028	255	pH 7.5, 37 °C	Open record: MurG, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurG	Escherichia coli	UDP-N-acetylglucosamine	0.72	0.027	26.5	pH 7.5, 37 °C	Open record: MurG, UDP-N-acetylglucosamine
MurGvariant E125A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.011	0.14	pH 7.5, 37 °C	Open record: MurG variant E125A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant E125A	Escherichia coli	UDP-N-acetylglucosamine	0.00155	0.012	0.128	pH 7.5, 37 °C	Open record: MurG variant E125A, UDP-N-acetylglucosamine
MurGvariant H124A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.009	0.18	pH 7.5, 37 °C	Open record: MurG variant H124A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant H124A	Escherichia coli	UDP-N-acetylglucosamine	0.00162	0.047	0.035	pH 7.5, 37 °C	Open record: MurG variant H124A, UDP-N-acetylglucosamine
MurGvariant N198A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0042	9.2	pH 7.5, 37 °C	Open record: MurG variant N198A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant N198A	Escherichia coli	UDP-N-acetylglucosamine	0.038	0.056	0.68	pH 7.5, 37 °C	Open record: MurG variant N198A, UDP-N-acetylglucosamine
MurGvariant N291A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0085	0.65	pH 7.5, 37 °C	Open record: MurG variant N291A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant N291A	Escherichia coli	UDP-N-acetylglucosamine	0.0055	0.0098	0.57	pH 7.5, 37 °C	Open record: MurG variant N291A, UDP-N-acetylglucosamine
MurGvariant R260A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0029	1.55	pH 7.5, 37 °C	Open record: MurG variant R260A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant R260A	Escherichia coli	UDP-N-acetylglucosamine	0.0045	0.033	0.137	pH 7.5, 37 °C	Open record: MurG variant R260A, UDP-N-acetylglucosamine
MurGvariant S191A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0071	13.2	pH 7.5, 37 °C	Open record: MurG variant S191A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant S191A	Escherichia coli	UDP-N-acetylglucosamine	0.093	1.03	0.09	pH 7.5, 37 °C	Open record: MurG variant S191A, UDP-N-acetylglucosamine
MurGvariant T15A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0055	0.18	pH 7.5, 37 °C	Open record: MurG variant T15A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant T15A	Escherichia coli	UDP-N-acetylglucosamine	0.00103	0.038	0.027	pH 7.5, 37 °C	Open record: MurG variant T15A, UDP-N-acetylglucosamine
MurGvariant Y105A	Escherichia coli	MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol	—	0.0059	20.8	pH 7.5, 37 °C	Open record: MurG variant Y105A, MurNAc(N-epsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol
MurGvariant Y105A	Escherichia coli	UDP-N-acetylglucosamine	0.123	0.05	2.5	pH 7.5, 37 °C	Open record: MurG variant Y105A, UDP-N-acetylglucosamine