Values CatLog read from this paper

Mechanism of poly(A) polymerase: structure of the enzyme-MgATP-RNA ternary complex and kinetic analysis

PMID 17850751doi:10.1016/j.str.2007.07.010Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (39 values) from this paper: poly(A) polymerase (PAP) from yeast.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.7.19

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
poly(A) polymerase (PAP)	yeast	18-mer oligoadenylate RNA (A18)	—	0.2592	—	30 °C	Open record: poly(A) polymerase (PAP), 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)	yeast	guanosine 5'-triphosphate	0.035	0.0617	0.57 calc.	30 °C	Open record: poly(A) polymerase (PAP), guanosine 5'-triphosphate
poly(A) polymerase (PAP)variant K215A	yeast	18-mer oligoadenylate RNA (A18)adenylyltransfer A_n + MgATP; A18 and MgATP varied	—	0.195	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant K215A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant K215A	yeast	18-mer oligoadenylate RNA (A18)cytidylyltransfer A_n + MgCTP; A18 and MgCTP varied	—	0.1299	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant K215A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant K215A	yeast	adenosine 5'-triphosphate (as MgATP2-)	3.18	0.4063	7.83 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant K215A, adenosine 5'-triphosphate (as MgATP2-)
poly(A) polymerase (PAP)variant K215A	yeast	cytidine 5'-triphosphate (as MgCTP2-)	0.0085	0.3685	0.023 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant K215A, cytidine 5'-triphosphate (as MgCTP2-)
poly(A) polymerase (PAP)variant N189A	yeast	18-mer oligoadenylate RNA (A18)adenylyltransfer A_n + MgATP; A18 and MgATP varied	—	0.106	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N189A	yeast	18-mer oligoadenylate RNA (A18)pyrophosphorolysis A_n + MgPPi; A18 and MgPPi varied	—	1.17	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N189A	yeast	18-mer oligoadenylate RNA (A18)cytidylyltransfer A_n + MgCTP; A18 and MgCTP varied	—	0.1456	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N189A	yeast	adenosine 5'-triphosphate (as MgATP2-)	11.08	0.08	140 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, adenosine 5'-triphosphate (as MgATP2-)
poly(A) polymerase (PAP)variant N189A	yeast	cytidine 5'-triphosphate (as MgCTP2-)	0.148	0.1483	1.00 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, cytidine 5'-triphosphate (as MgCTP2-)
poly(A) polymerase (PAP)variant N189A	yeast	inorganic pyrophosphate (as MgPPi2-)	0.91	0.721	1.3 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N189A, inorganic pyrophosphate (as MgPPi2-)
poly(A) polymerase (PAP)variant N226A	yeast	18-mer oligoadenylate RNA (A18)adenylyltransfer A_n + MgATP; A18 and MgATP varied	—	0.3672	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N226A	yeast	18-mer oligoadenylate RNA (A18)pyrophosphorolysis A_n + MgPPi; A18 and MgPPi varied	—	1.678	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N226A	yeast	18-mer oligoadenylate RNA (A18)cytidylyltransfer A_n + MgCTP; A18 and MgCTP varied	—	0.1318	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N226A	yeast	18-mer oligoadenylate RNA (A18)	—	0.2466	—	30 °C	Open record: poly(A) polymerase (PAP) variant N226A, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant N226A	yeast	adenosine 5'-triphosphate (as MgATP2-)	6.7	0.249	27 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, adenosine 5'-triphosphate (as MgATP2-)
poly(A) polymerase (PAP)variant N226A	yeast	cytidine 5'-triphosphate (as MgCTP2-)	0.0173	0.1406	0.123 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, cytidine 5'-triphosphate (as MgCTP2-)
poly(A) polymerase (PAP)variant N226A	yeast	guanosine 5'-triphosphate	0.0107	0.0549	0.190 calc.	30 °C	Open record: poly(A) polymerase (PAP) variant N226A, guanosine 5'-triphosphate
poly(A) polymerase (PAP)variant N226A	yeast	inorganic pyrophosphate (as MgPPi2-)	0.365	0.563	0.648 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant N226A, inorganic pyrophosphate (as MgPPi2-)
poly(A) polymerase (PAP)variant Y224F	yeast	18-mer oligoadenylate RNA (A18)adenylyltransfer A_n + MgATP; A18 and MgATP varied	—	0.7116	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant Y224F	yeast	18-mer oligoadenylate RNA (A18)pyrophosphorolysis A_n + MgPPi; A18 and MgPPi varied	—	0.121	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant Y224F	yeast	18-mer oligoadenylate RNA (A18)cytidylyltransfer A_n + MgCTP; A18 and MgCTP varied	—	3.5	—	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, 18-mer oligoadenylate RNA (A18)
poly(A) polymerase (PAP)variant Y224F	yeast	adenosine 5'-triphosphate (as MgATP2-)	15.07	0.9288	16.20 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, adenosine 5'-triphosphate (as MgATP2-)
poly(A) polymerase (PAP)variant Y224F	yeast	cytidine 5'-triphosphate (as MgCTP2-)	0.078	4.7	0.017 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, cytidine 5'-triphosphate (as MgCTP2-)
poly(A) polymerase (PAP)variant Y224F	yeast	inorganic pyrophosphate (as MgPPi2-)	0.063	0.375	0.17 calc.	pH 7, 30 °C	Open record: poly(A) polymerase (PAP) variant Y224F, inorganic pyrophosphate (as MgPPi2-)