Values CatLog read from this paper
Kinetic characterization of recombinant human cytosolic phosphoenolpyruvate carboxykinase with and without a His10-tag
PMID 17888579doi:10.1016/j.bbagen.2007.07.012Open in Browse
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CatLog read 10 records (10 values) from this paper: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | bicarbonate (supplied as KHCO3) | — | 20.6 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), bicarbonate (supplied as KHCO3) |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | bicarbonate (supplied as KHCO3)expressed in E. coli C41(DE3) from pET19b | — | 25.5 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), bicarbonate (supplied as KHCO3) |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | guanosine 5'-diphosphate | — | 0.0787 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), guanosine 5'-diphosphate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | guanosine 5'-diphosphateexpressed in E. coli C41(DE3) from pET19b | — | 0.0921 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), guanosine 5'-diphosphate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | guanosine 5'-triphosphate | — | 0.0234 | — | pH 8, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), guanosine 5'-triphosphate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | guanosine 5'-triphosphateexpressed in E. coli C41(DE3) from pET19b | — | 0.0222 | — | pH 8, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), guanosine 5'-triphosphate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | oxaloacetate | — | 0.004 | — | pH 8, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), oxaloacetate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | oxaloacetateexpressed in E. coli C41(DE3) from pET19b | — | 0.0055 | — | pH 8, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), oxaloacetate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | phosphoenolpyruvate | — | 0.45 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), phosphoenolpyruvate |
| cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK) | Homo sapiens | phosphoenolpyruvateexpressed in E. coli C41(DE3) from pET19b | — | 0.237 | — | pH 7.2, 37 °C | Open record: cytosolic GTP-dependent phosphoenolpyruvate carboxykinase (cPEPCK, GTP-PEPCK), phosphoenolpyruvate |