Values CatLog read from this paper
Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants
PMID 17894826doi:10.1111/j.1742-4658.2007.06059.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: fructose-1,6-bisphosphatase (FBPase) from Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| fructose-1,6-bisphosphatase (FBPase) | Sus scrofa | fructose-1,6-bisphosphatepurified from pig kidney (nonrecombinant enzyme) | 20.7 | 0.0059 | 3.5 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase), fructose-1,6-bisphosphate |
| fructose-1,6-bisphosphatase (FBPase) | Sus scrofa | fructose-1,6-bisphosphaterecombinant His-FBPase expressed from pET15b | 19.7 | 0.0048 | 4.1 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase), fructose-1,6-bisphosphate |
| fructose-1,6-bisphosphatase (FBPase)variant F16W | Sus scrofa | fructose-1,6-bisphosphate | 18.1 | 0.005 | 3.6 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase) variant F16W, fructose-1,6-bisphosphate |
| fructose-1,6-bisphosphatase (FBPase)variant F219W | Sus scrofa | fructose-1,6-bisphosphate | 18.7 | 0.006 | 3.1 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase) variant F219W, fructose-1,6-bisphosphate |
| fructose-1,6-bisphosphatase (FBPase)variant F232W | Sus scrofa | fructose-1,6-bisphosphate | 14.2 | 0.0046 | 3.1 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase) variant F232W, fructose-1,6-bisphosphate |
| fructose-1,6-bisphosphatase (FBPase)variant F89W | Sus scrofa | fructose-1,6-bisphosphate | 19.2 | 0.0043 | 4.5 × 10³ calc. | pH 7.5, 30 °C | Open record: fructose-1,6-bisphosphatase (FBPase) variant F89W, fructose-1,6-bisphosphate |