Values CatLog read from this paper
Investigation of the Methanosarcina thermophila acetate kinase mechanism by fluorescence quenching
PMID 17999468doi:10.1021/bi701292aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (29 values) from this paper: acetate kinase from Methanosarcina thermophila.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetate kinasevariant Q43W | Methanosarcina thermophila | acetate | 699 | 12.4 | 56.4 calc. | Open record: acetate kinase variant Q43W, acetate | |
| acetate kinasevariant Q43W | Methanosarcina thermophila | acetyl phosphate | 2.65 × 10³ | 0.26 | 1.0 × 10⁴ calc. | Open record: acetate kinase variant Q43W, acetyl phosphate | |
| acetate kinasevariant Q43W | Methanosarcina thermophila | ADP·Mg | — | 0.173 | — | Open record: acetate kinase variant Q43W, ADP·Mg | |
| acetate kinasevariant Q43W | Methanosarcina thermophila | ATP·Mg | — | 0.053 | — | Open record: acetate kinase variant Q43W, ATP·Mg | |
| acetate kinasevariant R241A/Q43W | Methanosarcina thermophila | acetate | 1.83 | 5.8 | 0.32 calc. | Open record: acetate kinase variant R241A/Q43W, acetate | |
| acetate kinasevariant R241A/Q43W | Methanosarcina thermophila | acetyl phosphate | 14.6 | 0.33 | 44 calc. | Open record: acetate kinase variant R241A/Q43W, acetyl phosphate | |
| acetate kinasevariant R241A/Q43W | Methanosarcina thermophila | ADP·Mg | — | 0.239 | — | Open record: acetate kinase variant R241A/Q43W, ADP·Mg | |
| acetate kinasevariant R241A/Q43W | Methanosarcina thermophila | ATP·Mg | — | 0.0805 | — | Open record: acetate kinase variant R241A/Q43W, ATP·Mg | |
| acetate kinasevariant R241K/Q43W | Methanosarcina thermophila | acetate | 0.4 | 179 | 0.0022 calc. | Open record: acetate kinase variant R241K/Q43W, acetate | |
| acetate kinasevariant R241K/Q43W | Methanosarcina thermophila | acetyl phosphate | 10.5 | 1.02 | 10.3 calc. | Open record: acetate kinase variant R241K/Q43W, acetyl phosphate | |
| acetate kinasevariant R241K/Q43W | Methanosarcina thermophila | ADP·Mg | — | 0.692 | — | Open record: acetate kinase variant R241K/Q43W, ADP·Mg | |
| acetate kinasevariant R241K/Q43W | Methanosarcina thermophila | ATP·Mg | — | 1.3 | — | Open record: acetate kinase variant R241K/Q43W, ATP·Mg | |
| acetate kinasevariant R91A/Q43W | Methanosarcina thermophila | acetate | 2.07 | — | — | Open record: acetate kinase variant R91A/Q43W, acetate | |
| acetate kinasevariant R91A/Q43W | Methanosarcina thermophila | acetyl phosphate | 2.63 | 2.58 | 1.02 calc. | Open record: acetate kinase variant R91A/Q43W, acetyl phosphate | |
| acetate kinasevariant R91A/Q43W | Methanosarcina thermophila | ADP·Mg | — | 0.147 | — | Open record: acetate kinase variant R91A/Q43W, ADP·Mg | |
| acetate kinasevariant R91A/Q43W | Methanosarcina thermophila | ATP·Mg | — | 0.539 | — | Open record: acetate kinase variant R91A/Q43W, ATP·Mg | |
| acetate kinasevariant R91K/Q43W | Methanosarcina thermophila | acetate | 11.8 | 48 | 0.25 calc. | Open record: acetate kinase variant R91K/Q43W, acetate | |
| acetate kinasevariant R91K/Q43W | Methanosarcina thermophila | acetyl phosphate | 25.7 | 1.08 | 23.8 calc. | Open record: acetate kinase variant R91K/Q43W, acetyl phosphate | |
| acetate kinasevariant R91K/Q43W | Methanosarcina thermophila | ADP·Mg | — | 0.164 | — | Open record: acetate kinase variant R91K/Q43W, ADP·Mg | |
| acetate kinasevariant R91K/Q43W | Methanosarcina thermophila | ATP·Mg | — | 0.234 | — | Open record: acetate kinase variant R91K/Q43W, ATP·Mg |