Values CatLog read from this paper
Directed evolution of rubisco in Escherichia coli reveals a specificity-determining hydrogen bond in the form II enzyme
PMID 18004873doi:10.1021/bi700820aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (35 values) from this paper: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) from Rhodospirillum rubrum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) | Rhodospirillum rubrum | CO2 | 12.3 | 0.149 | 83 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), CO2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) | Rhodospirillum rubrum | D-ribulose-1,5-bisphosphate | — | 0.063 | — | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), D-ribulose-1,5-bisphosphate |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) | Rhodospirillum rubrum | O2 | 1.4 | 0.159 | 8.9 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), O2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117H | Rhodospirillum rubrum | CO2 | 7.5 | 0.192 | 39 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117H, CO2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117H | Rhodospirillum rubrum | D-ribulose-1,5-bisphosphate | — | 0.048 | — | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117H, D-ribulose-1,5-bisphosphate |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117H | Rhodospirillum rubrum | O2 | 1.1 | 0.153 | 7.3 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117H, O2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117V | Rhodospirillum rubrum | CO2 | 7.4 | 0.196 | 38 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117V, CO2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117V | Rhodospirillum rubrum | D-ribulose-1,5-bisphosphate | — | 0.055 | — | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117V, D-ribulose-1,5-bisphosphate |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant D117V | Rhodospirillum rubrum | O2 | 1.5 | 0.199 | 7.6 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant D117V, O2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44N | Rhodospirillum rubrum | CO2 | 9.8 | 0.204 | 48 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44N, CO2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44N | Rhodospirillum rubrum | D-ribulose-1,5-bisphosphate | — | 0.059 | — | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44N, D-ribulose-1,5-bisphosphate |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44N | Rhodospirillum rubrum | O2 | 1 | 0.116 | 8.8 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44N, O2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44Q | Rhodospirillum rubrum | CO2 | 9.3 | 0.301 | 31 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44Q, CO2 |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44Q | Rhodospirillum rubrum | D-ribulose-1,5-bisphosphate | — | 0.056 | — | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44Q, D-ribulose-1,5-bisphosphate |
| Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase)variant H44Q | Rhodospirillum rubrum | O2 | 1.2 | 0.185 | 6.4 | pH 8, 25 °C | Open record: Form II Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) variant H44Q, O2 |