CatLogenzyme kinetics catalog

Values CatLog read from this paper

The role of OXA-1 beta-lactamase Asp(66) in the stabilization of the active-site carbamate group and in substrate turnover

PMID 18031291doi:10.1042/bj20070573Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 31 records (91 values) from this paper: OXA-1 beta-lactamase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.2.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
OXA-1 beta-lactamase—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid1.60.0024700pH 7.4Open
OXA-1 beta-lactamase—Ampicillinuntagged (pETKMOXA-1 in E. coli BL21(DE3))4400.0143.1 × 10⁴ calc.pH 7.4Open
OXA-1 beta-lactamase—AmpicillinN-terminal His6-tag fusion peptide (pET-28a(+))5200.0212.5 × 10⁴pH 7.4Open
OXA-1 beta-lactamase—Cefepime340.17200pH 7.4Open
OXA-1 beta-lactamase—Cefotaximeuntagged (pETKMOXA-1 in E. coli BL21(DE3))6.70.029230 calc.pH 7.4Open
OXA-1 beta-lactamase—CefotaximeN-terminal His6-tag fusion peptide (pET-28a(+))5.30.036150pH 7.4Open
OXA-1 beta-lactamase—Cephalothin2.70.009300pH 7.4Open
OXA-1 beta-lactamasevariant D66A—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid0.190.01315pH 7.4Open
OXA-1 beta-lactamasevariant D66A—Ampicillin130.0062.1 × 10³pH 7.4Open
OXA-1 beta-lactamasevariant D66A—Cefepime0.550.02720pH 7.4Open
OXA-1 beta-lactamasevariant D66A—Cefotaxime0.370.075pH 7.4Open
OXA-1 beta-lactamasevariant D66A—Cephalothin0.270.13pH 7.4Open
OXA-1 beta-lactamasevariant D66M—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid0.170.003250pH 7.4Open
OXA-1 beta-lactamasevariant D66M—Ampicillin430.0123.5 × 10³pH 7.4Open
OXA-1 beta-lactamasevariant D66M—Cefepime0.430.03512pH 7.4Open
OXA-1 beta-lactamasevariant D66M—Cefotaxime0.310.0369pH 7.4Open
OXA-1 beta-lactamasevariant D66M—Cephalothin0.380.0140pH 7.4Open
OXA-1 beta-lactamasevariant D66N—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid1.20.002600pH 7.4Open
OXA-1 beta-lactamasevariant D66N—Ampicillin4800.0232.1 × 10⁴pH 7.4Open
OXA-1 beta-lactamasevariant D66N—Cefepime270.15180pH 7.4Open
OXA-1 beta-lactamasevariant D66N—Cefotaxime3.50.024150pH 7.4Open
OXA-1 beta-lactamasevariant D66N—Cephalothin1.60.011150pH 7.4Open
OXA-1 beta-lactamasevariant D66W—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid0.080.00614pH 7.4Open
OXA-1 beta-lactamasevariant D66W—Ampicillin560.0212.7 × 10³pH 7.4Open
OXA-1 beta-lactamasevariant D66W—Cefepime0.0080.210.04pH 7.4Open
OXA-1 beta-lactamasevariant D66W—Cephalothin0.150.0593pH 7.4Open
OXA-1 beta-lactamasevariant D66Y—(6R,7S)-3-[(3-carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid0.250.00734pH 7.4Open
OXA-1 beta-lactamasevariant D66Y—Ampicillin10.60.00273.9 × 10³pH 7.4Open
OXA-1 beta-lactamasevariant D66Y—Cefepime3.80.2317pH 7.4Open
OXA-1 beta-lactamasevariant D66Y—Cefotaxime0.710.05712pH 7.4Open
OXA-1 beta-lactamasevariant D66Y—Cephalothin0.280.0378pH 7.4Open