Values CatLog read from this paper
Mechanism of the insect enzyme, tyramine beta-monooxygenase, reveals differences from the mammalian enzyme, dopamine beta-monooxygenase
PMID 18032384doi:10.1074/jbc.m705911200Open in Browse
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CatLog read 35 records (103 values) from this paper: tyramine β-monooxygenase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 56 µM O2 | 1.14 | 0.231 | 4.95 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 119 µM O2 | 1.93 | 0.177 | 10.9 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 215 µM O2 | 2.55 | 0.178 | 14.3 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 395 µM O2 | 2.8 | 0.145 | 19.4 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 587 µM O2 | 4.08 | 0.203 | 20.1 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)10 mM ascorbate; 907 µM O2 | 3.57 | 0.0906 | 39.4 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 63 µM O2 | 2 | 0.341 | 5.8 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 110 µM O2 | 2.5 | 0.257 | 9.6 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 214 µM O2 | 3.3 | 0.245 | 13.5 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 416 µM O2 | 6.37 | 0.328 | 19.4 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 598 µM O2 | 7.8 | 0.316 | 24.6 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 907 µM O2 | 6.8 | 0.233 | 29.2 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine)50 mM ascorbate; 1039 µM O2 | 7.45 | 0.193 | 38.6 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, 4-hydroxy-β,β-[2-2H2]phenethylamine (β,β-dideuterated tyramine) |
| tyramine β-monooxygenase | — | ascorbate50 µM tyramine fixed | 1.28 | 0.93 | 1.38 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, ascorbate |
| tyramine β-monooxygenase | — | ascorbate100 µM tyramine fixed | 2.27 | 1.98 | 1.14 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, ascorbate |
| tyramine β-monooxygenase | — | ascorbatefit to Michaelis-Menten equation (no K_i term) | 4.52 | 5.6 | 0.8 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, ascorbate |
| tyramine β-monooxygenase | — | ascorbatefit to Michaelis-Menten equation (no K_i term) | 8.74 | 16 | 0.55 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, ascorbate |
| tyramine β-monooxygenase | — | dioxygen | — | 0.35 | — | pH 6, 35 °C | Open record: tyramine β-monooxygenase, dioxygen |
| tyramine β-monooxygenase | — | tyramine2 mM ascorbate; air-saturated (222 µM O2) | 1.31 | 0.0252 | 52 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine4 mM ascorbate; air-saturated (222 µM O2) | 3.2 | 0.096 | 33 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; air-saturated (222 µM O2) | 3.91 | 0.088 | 45 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; air-saturated (222 µM O2) | 8.9 | 0.235 | 37.8 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine100 mM ascorbate; air-saturated (222 µM O2) | 11.9 | 0.367 | 32.4 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine200 mM ascorbate; air-saturated (222 µM O2) | 8.5 | 0.265 | 31.9 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; 58 µM O2; cosubstrate ascorbate; O2 | 1.9 | 0.094 | 20.7 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; 103 µM O2; cosubstrate ascorbate; O2 | 2.9 | 0.113 | 25.5 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; 512 µM O2; cosubstrate ascorbate; O2 | 5.7 | 0.098 | 58.1 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; 885 µM O2; cosubstrate ascorbate; O2 | 9.8 | 0.159 | 61.7 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine10 mM ascorbate; 1040 µM O2; cosubstrate ascorbate; O2 | 7.25 | 0.0976 | 74.3 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 59 µM O2; cosubstrate ascorbate; O2 | 2.86 | 0.188 | 15.4 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 99 µM O2; cosubstrate ascorbate; O2 | 5.86 | 0.215 | 27.2 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 395 µM O2; cosubstrate ascorbate; O2 | 11.5 | 0.242 | 47.7 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 608 µM O2; cosubstrate ascorbate; O2 | 15 | 0.256 | 58.6 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 854 µM O2; cosubstrate ascorbate; O2 | 18.6 | 0.387 | 48.2 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |
| tyramine β-monooxygenase | — | tyramine50 mM ascorbate; 1040 µM O2; cosubstrate ascorbate; O2 | 16.1 | 0.23 | 70 | pH 6, 35 °C | Open record: tyramine β-monooxygenase, tyramine |